CPLM-009840

Genbank Nucleotide ID

Guanine nucleotide-binding protein subunit beta-2-like 1

Protein Synonyms/Alias

12-3; Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1; p205

Mus musculus (Mouse)

Position	Peptide	Type	References
12	MTLRGTLKGHNGWVT	ubiquitination	[1]
106	RRFVGHTKDVLSVAF	ubiquitination	[1]
130	GSRDKTIKLWNTLGV	acetylation	[2, 3, 4, 5, 6, 7]
130	GSRDKTIKLWNTLGV	ubiquitination	[1]
172	IVSCGWDKLVKVWNL	ubiquitination	[1]
175	CGWDKLVKVWNLANC	acetylation	[3, 6]
175	CGWDKLVKVWNLANC	succinylation	[6]
175	CGWDKLVKVWNLANC	ubiquitination	[1]
183	VWNLANCKLKTNHIG	acetylation	[3, 4, 5, 6, 7]
183	VWNLANCKLKTNHIG	succinylation	[6]
183	VWNLANCKLKTNHIG	ubiquitination	[1]
257	AATGPSIKIWDLEGK	ubiquitination	[1]
271	KIIVDELKQEVISTS	acetylation	[3, 4, 6]
271	KIIVDELKQEVISTS	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand- independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration.

REPEAT 13 44 WD 1.
REPEAT 61 91 WD 2.
REPEAT 103 133 WD 3.
REPEAT 146 178 WD 4.
REPEAT 190 220 WD 5.
REPEAT 231 260 WD 6.
REPEAT 281 311 WD 7.
MOD_RES 2 2 N-acetylthreonine.
MOD_RES 52 52 Phosphotyrosine; by ABL1 (By similarity).
MOD_RES 130 130 N6-acetyllysine (By similarity).
MOD_RES 228 228 Phosphotyrosine (By similarity).

Acetylation; Apoptosis; Biological rhythms; Cell cycle; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Direct protein sequencing; Gastrulation; Growth regulation; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; Translation regulation; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
GO:0030425; C:dendrite; IDA:UniProtKB.
GO:0030496; C:midbody; ISS:UniProtKB.
GO:0043025; C:neuronal cell body; IDA:MGI.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
GO:0001891; C:phagocytic cup; ISS:UniProtKB.
GO:0015935; C:small ribosomal subunit; IEA:Compara.
GO:0008656; F:cysteine-type endopeptidase activator activity involved in apoptotic process; IEA:Compara.
GO:0008200; F:ion channel inhibitor activity; IDA:UniProtKB.
GO:0005080; F:protein kinase C binding; ISS:UniProtKB.
GO:0030292; F:protein tyrosine kinase inhibitor activity; ISS:UniProtKB.
GO:0004872; F:receptor activity; ISA:MGI.
GO:0030971; F:receptor tyrosine kinase binding; ISS:UniProtKB.
GO:0042169; F:SH2 domain binding; ISS:UniProtKB.
GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
GO:0007369; P:gastrulation; IEA:UniProtKB-KW.
GO:0035556; P:intracellular signal transduction; ISA:MGI.
GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
GO:0050765; P:negative regulation of phagocytosis; ISS:UniProtKB.
GO:0051898; P:negative regulation of protein kinase B signaling cascade; IEA:Compara.
GO:0017148; P:negative regulation of translation; IEA:Compara.
GO:0030178; P:negative regulation of Wnt receptor signaling pathway; ISS:UniProtKB.
GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
GO:0030335; P:positive regulation of cell migration; ISS:UniProtKB.
GO:2000543; P:positive regulation of gastrulation; ISS:UniProtKB.
GO:0043547; P:positive regulation of GTPase activity; ISS:UniProtKB.
GO:2001244; P:positive regulation of intrinsic apoptotic signaling pathway; IEA:Compara.
GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
GO:0032464; P:positive regulation of protein homooligomerization; ISS:UniProtKB.
GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
GO:0008104; P:protein localization; ISA:MGI.
GO:0051726; P:regulation of cell cycle; ISS:UniProtKB.
GO:0051302; P:regulation of cell division; ISS:UniProtKB.
GO:2000114; P:regulation of establishment of cell polarity; ISS:UniProtKB.
GO:0090003; P:regulation of establishment of protein localization to plasma membrane; IEA:Compara.
GO:0032880; P:regulation of protein localization; ISS:UniProtKB.
GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.

IPR020472; G-protein_beta_WD-40_rep.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00320; GPROTEINBRPT.