UniProt Accession

 LIMA1_MOUSE; Q9ERG0; Q8K2H0; Q9ERG1 

Genbank Protein ID

 AF307844; AF307845; BC031490; CH466550 

Genbank Nucleotide ID

 AAG31147.1; AAG31148.1; AAH31490.1; EDL04110.1 

Protein Name

 LIM domain and actin-binding protein 1 

Protein Synonyms/Alias

 Epithelial protein lost in neoplasm; mEPLIN 

Gene Name

 Lima1 

Gene Synonyms/Alias

 D15Ertd366e; Eplin 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
189	ETSGKIEKYNVPLNR	acetylation	[1]
268	RLSETSIKDRMAKYQ	ubiquitination	[2]
437	LHGRIYCKPHFNQLF	acetylation	[1]
437	LHGRIYCKPHFNQLF	succinylation	[1]
719	EFTTQNQKSQDVGFW	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). 

Sequence Annotation

 DOMAIN 386 446 LIM zinc-binding.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 15 15 Phosphoserine (By similarity).
 MOD_RES 132 132 Phosphoserine (By similarity).
 MOD_RES 225 225 Phosphoserine.
 MOD_RES 230 230 Phosphoserine.
 MOD_RES 263 263 Phosphoserine (By similarity).
 MOD_RES 348 348 Phosphoserine (By similarity).
 MOD_RES 360 360 Phosphoserine (By similarity).
 MOD_RES 367 367 Phosphoserine.
 MOD_RES 372 372 Phosphoserine (By similarity).
 MOD_RES 467 467 Phosphoserine.
 MOD_RES 485 485 Phosphoserine.
 MOD_RES 488 488 Phosphoserine.
 MOD_RES 539 539 Phosphoserine.
 MOD_RES 602 602 Phosphoserine (By similarity).
 MOD_RES 607 607 Phosphoserine (By similarity).
 MOD_RES 615 615 Phosphoserine.
 MOD_RES 692 692 Phosphoserine (By similarity).
 MOD_RES 720 720 Phosphoserine (By similarity).
 MOD_RES 735 735 Phosphoserine.  

Keyword

 Acetylation; Actin-binding; Alternative promoter usage; Complete proteome; Cytoplasm; Cytoskeleton; LIM domain; Metal-binding; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 753 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0001725; C:stress fiber; ISS:UniProtKB.
 GO:0051015; F:actin filament binding; ISS:UniProtKB.
 GO:0003785; F:actin monomer binding; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; ISS:UniProtKB.
 GO:0030835; P:negative regulation of actin filament depolymerization; ISS:UniProtKB.
 GO:0031529; P:ruffle organization; ISS:UniProtKB. 

Interpro

 IPR001781; Znf_LIM. 

Pfam

 PF00412; LIM 

SMART

 SM00132; LIM 

PROSITE

 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2 

PRINTS