CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022029
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Xaa-Pro aminopeptidase 1 
Protein Synonyms/Alias
 Aminoacylproline aminopeptidase; Cytosolic aminopeptidase P; Soluble aminopeptidase P; sAmp; X-Pro aminopeptidase 1; X-prolyl aminopeptidase 1, soluble 
Gene Name
 XPNPEP1 
Gene Synonyms/Alias
 XPNPEPL; XPNPEPL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84RYFLQAAKQMDSNWTubiquitination[1]
130IIPTDYWKKMAKVLRacetylation[2]
130IIPTDYWKKMAKVLRubiquitination[3]
131IPTDYWKKMAKVLRSubiquitination[1]
287ADLSPREKVWVSDKAubiquitination[1]
304AVSETIPKDHRCCMPacetylation[2]
319YTPICIAKAVKNSAEubiquitination[1]
336GMRRAHIKDAVALCEubiquitination[1]
365TEISAADKAEEFRRQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. 
Sequence Annotation
 METAL 415 415 Manganese 1.
 METAL 426 426 Manganese 1.
 METAL 426 426 Manganese 2.
 METAL 489 489 Manganese 2.
 METAL 523 523 Manganese 2.
 METAL 537 537 Manganese 1.
 METAL 537 537 Manganese 2.
 MOD_RES 304 304 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Aminopeptidase; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Manganese; Metal-binding; Metalloprotease; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 623 AA 
Protein Sequence
MPPKVTSELL RQLRQAMRNS EYVTEPIQAY IIPSGDAHQS EYIAPCDCRR AFVSGFDGSA 60
GTAIITEEHA AMWTDGRYFL QAAKQMDSNW TLMKMGLKDT PTQEDWLVSV LPEGSRVGVD 120
PLIIPTDYWK KMAKVLRSAG HHLIPVKENL VDKIWTDRPE RPCKPLLTLG LDYTGISWKD 180
KVADLRLKMA ERNVMWFVVT ALDEIAWLFN LRGSDVEHNP VFFSYAIIGL ETIMLFIDGD 240
RIDAPSVKEH LLLDLGLEAE YRIQVHPYKS ILSELKALCA DLSPREKVWV SDKASYAVSE 300
TIPKDHRCCM PYTPICIAKA VKNSAESEGM RRAHIKDAVA LCELFNWLEK EVPKGGVTEI 360
SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPVP ETNRTLSLDE VYLIDSGAQY 420
KDGTTDVTRT MHFGTPTAYE KECFTYVLKG HIAVSAAVFP TGTKGHLLDS FARSALWDSG 480
LDYLHGTGHG VGSFLNVHEG PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV 540
LVVPVKTKYN FNNRGSLTFE PLTLVPIQTK MIDVDSLTDK ECDWLNNYHL TCRDVIGKEL 600
QKQGRQEALE WLIRETQPIS KQH 623 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0030145; F:manganese ion binding; IDA:UniProtKB.
 GO:0070006; F:metalloaminopeptidase activity; IDA:UniProtKB.
 GO:0010815; P:bradykinin catabolic process; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IDA:UniProtKB. 
Interpro
 IPR000587; Creatinase.
 IPR000994; Pept_M24_structural-domain.
 IPR001131; Peptidase_M24B_aminopep-P_CS. 
Pfam
 PF01321; Creatinase_N
 PF00557; Peptidase_M24 
SMART
  
PROSITE
 PS00491; PROLINE_PEPTIDASE 
PRINTS