CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038660
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Methionine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 Mars 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
191RAAETVLKQQGVLALubiquitination[1]
253LESLPPLKLQQHPVLubiquitination[1]
337TPREICDKYHAIHADubiquitination[1]
366TTTPQQTKITQDIFQubiquitination[1]
433LINAIELKKPQCKICubiquitination[1]
502RCITRDLKWGTPVPLubiquitination[1]
595YLNYEDGKFSKSRGIubiquitination[1]
598YEDGKFSKSRGIGVFubiquitination[1]
728IQVNEPWKRIKGGEMacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MRLFVSEGSP GSLPVLAAAA RARGRAELLI STVGPEECVV PFLTRPKVPV LQLDSGNYLF 60
SASAICRYFF LLCGWEQDDL TNQWLEWEAT ELQPVLSAAL HCLVVQGKKG EDILGPLRRV 120
LTHIDHSLSR QNCPFLAGDT ESLADIVLWG ALYPLLQDPA YLPEELGALQ SWFQTLSTQE 180
PCQRAAETVL KQQGVLALRL YLQKQPQPQP PPPEGRTVSN ELEEEELATL SEEDIVTAVA 240
AWEKGLESLP PLKLQQHPVL PVPGERNVLI TSALPYVNNV PHLGNIIGCV LSADVFARYC 300
RLRQWNTLYL CGTDEYGTAT ETKAMEEGLT PREICDKYHA IHADIYRWFG ISFDTFGRTT 360
TPQQTKITQD IFQRLLTRGF VLRDTVEQLR CERCARFLAD RFVEGVCPFC GYEEARGDQC 420
DRCGKLINAI ELKKPQCKIC RSCPVVRSSQ HLFLDLPKLE KRLEDWLGKT VPGSDWTPNA 480
RFIIRSWLRD GLKPRCITRD LKWGTPVPLE GFEDKVFYVW FDATIGYVSI TANYTDQWEK 540
WWKNPEQVDL YQFMAKDNVP FHGLVFPCSV LGAEDNYTLV KHIIATEYLN YEDGKFSKSR 600
GIGVFGDMAK DTGIPADIWR FYLLYIRPEG QDSAFSWTDL LIKNNSELLN NLGNFINRAG 660
MFVSKFFGGC VPEMALTPDD RRLVAHVSWE LQHYHQLLEK VRIRDALRSI LTISRHGNQY 720
IQVNEPWKRI KGGEMDRQRA GTVTGMAVNM AALLSVMLQP YMPTVSSTIQ TQLQLPPAAC 780
RILATSFICT LPAGHRIGTV SPLFQKLEND QIENLRQRFG GGQLEESLEL KAKGSPKPAA 840
VEAVTAAGSQ HIQTLTDEVT KQGNVVRELK AQKADKNQVA AEVAKLLDLK KQLALAEGKP 900
IETPKGKKKK 910 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004825; F:methionine-tRNA ligase activity; IEA:InterPro.
 GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR010987; Glutathione-S-Trfase_C-like.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR023458; Met-tRNA_ligase_1.
 IPR014758; Met-tRNA_synth.
 IPR015413; Methionyl/Leucyl_tRNA_Synth.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009068; S15_NS1_RNA-bd.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR000738; WHEP-TRS. 
Pfam
 PF09334; tRNA-synt_1g
 PF00458; WHEP-TRS 
SMART
 SM00991; WHEP-TRS 
PROSITE
 PS00178; AA_TRNA_LIGASE_I
 PS50405; GST_CTER
 PS00762; WHEP_TRS_1
 PS51185; WHEP_TRS_2 
PRINTS
 PR01041; TRNASYNTHMET.