UniProt Accession

 EI3JB_MOUSE; Q66JS6; Q8BUW6 

Genbank Protein ID

 BC080788; AK082048 

Genbank Nucleotide ID

 AAH80788.1; BAC38399.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit J-B 

Protein Synonyms/Alias

 eIF3j-B; Eukaryotic translation initiation factor 3 subunit 1-B; eIF-3-alpha-B; eIF3 p35 

Gene Name

 Eif3j2 

Gene Synonyms/Alias

 Eif3s1-2; Gm9781 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
122	PEEQLADKLRLKKLQ	ubiquitination	[1]
166	DDFTEFGKLLKDKIT	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA (By similarity). 

Sequence Annotation

 REGION 6 74 Sufficient for interaction with EIF3B (By
 REGION 248 263 Promotes stable association with the 40S
 MOD_RES 16 16 Phosphoserine (By similarity).
 MOD_RES 18 18 Phosphoserine (By similarity).
 MOD_RES 25 25 Phosphoserine (By similarity).
 MOD_RES 114 114 Phosphothreonine (By similarity).
 MOD_RES 132 132 Phosphoserine (By similarity).
 MOD_RES 259 259 Phosphotyrosine (By similarity).  

Keyword

 Coiled coil; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 263 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 

Interpro

 IPR023194; eIF3-like_dom.
 IPR013906; eIF3j. 

Pfam

 PF08597; eIF3_subunit 

SMART

  

PROSITE

  

PRINTS