CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000919
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma 
Protein Synonyms/Alias
 PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; Phosphatidylinositol 4-phosphate 5-kinase type I gamma; PIP5KIgamma 
Gene Name
 PIP5K1C 
Gene Synonyms/Alias
 KIAA0589 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
97TVGHLSSKPERDVLMubiquitination[1, 2]
200KEAEFLQKLLPGYYMubiquitination[1]
351EKRPVGQKALYSTAMubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A have a role during embryogenesis and together with PIP5K1B may have a role immediately after birth. 
Sequence Annotation
 DOMAIN 75 443 PIPK.
 REGION 641 668 Mediates interaction with TLN2.
 MOD_RES 265 265 N6-acetyllysine.
 MOD_RES 268 268 N6-acetyllysine.
 MOD_RES 639 639 Phosphotyrosine; by EGFR (By similarity).
 MOD_RES 649 649 Phosphotyrosine; by CSK.
 MOD_RES 650 650 Phosphoserine; by CDK5, MAPK1 and CDK1.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell adhesion; Cell junction; Cell membrane; Cell projection; Chemotaxis; Complete proteome; Cytoplasm; Disease mutation; Endocytosis; Exocytosis; Kinase; Membrane; Nucleotide-binding; Nucleus; Phagocytosis; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 668 AA 
Protein Sequence
MELEVPDEAE SAEAGAVPSE AAWAAESGAA AGLAQKKAAP TEVLSMTAQP GPGHGKKLGH 60
RGVDASGETT YKKTTSSTLK GAIQLGIGYT VGHLSSKPER DVLMQDFYVV ESIFFPSEGS 120
NLTPAHHFQD FRFKTYAPVA FRYFRELFGI RPDDYLYSLC NEPLIELSNP GASGSLFYVT 180
SDDEFIIKTV MHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCVQSGG KNIRVVVMNN 240
ILPRVVKMHL KFDLKGSTYK RRASKKEKEK SFPTYKDLDF MQDMPEGLLL DADTFSALVK 300
TLQRDCLVLE SFKIMDYSLL LGVHNIDQHE RERQAQGAQS TSDEKRPVGQ KALYSTAMES 360
IQGGAARGEA IESDDTMGGI PAVNGRGERL LLHIGIIDIL QSYRFIKKLE HTWKALVHDG 420
DTVSVHRPSF YAERFFKFMS NTVFRKNSSL KSSPSKKGRG GALLAVKPLG PTAAFSASQI 480
PSEREEAQYD LRGARSYPTL EDEGRPDLLP CTPPSFEEAT TASIATTLSS TSLSIPERSP 540
SETSEQPRYR RRTQSSGQDG RPQEEPPAEE DLQQITVQVE PACSVEIVVP KEEDAGVEAS 600
PAGASAAVEV ETASQASDEE GAPASQASDE EDAPATDIYF FTDGRYWIYS PRHRRLRAVT 660
LSASGTVSDR SRPPWGEGAV PLGQQGAAGP RPEAQCLTSV VFQKGFG 707 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0005925; C:focal adhesion; TAS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0001891; C:phagocytic cup; IEA:UniProtKB-SubCell.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0001931; C:uropod; TAS:UniProtKB.
 GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030036; P:actin cytoskeleton organization; TAS:UniProtKB.
 GO:0034333; P:adherens junction assembly; TAS:UniProtKB.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0016337; P:cell-cell adhesion; TAS:UniProtKB.
 GO:0072583; P:clathrin-mediated endocytosis; TAS:UniProtKB.
 GO:0030593; P:neutrophil chemotaxis; TAS:UniProtKB.
 GO:0006909; P:phagocytosis; TAS:UniProtKB.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0046854; P:phosphatidylinositol phosphorylation; IEA:GOC.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0048488; P:synaptic vesicle endocytosis; TAS:UniProtKB.
 GO:0016079; P:synaptic vesicle exocytosis; TAS:UniProtKB. 
Interpro
 IPR023610; PInositol-4-P-5-kinase.
 IPR027483; PInositol-4-P-5-kinase_C.
 IPR002498; PInositol-4-P-5-kinase_core.
 IPR027484; PInositol-4-P-5-kinase_N.
 IPR016034; PInositol-4P-5-kinase_core_sub. 
Pfam
 PF01504; PIP5K 
SMART
 SM00330; PIPKc 
PROSITE
 PS51455; PIPK 
PRINTS