| Tag | Content |
|---|
| CPLM ID | CPLM-017565 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Lysine-specific histone demethylase 1B |
Protein Synonyms/Alias | Flavin-containing amine oxidase domain-containing protein 1; Lysine-specific histone demethylase 2 |
Gene Name | KDM1B |
Gene Synonyms/Alias | AOF1; C6orf193; LSD2 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 109 | SHKDGYDKYTTWKKI | ubiquitination | [1] | | 155 | RKWRQLTKEIQLTPQ | ubiquitination | [2] | | 165 | QLTPQIAKTYRCGMK | ubiquitination | [2] | | 250 | TGNASPGKLEHSKAA | ubiquitination | [2] | | 378 | ADQYLLPKDYHNKSV | ubiquitination | [1] | | 414 | KVTVLEAKDRIGGRV | ubiquitination | [2] | | 425 | GGRVWDDKSFKGVTV | ubiquitination | [2] | | 428 | VWDDKSFKGVTVGRG | ubiquitination | [2] | | 481 | ITDPTIDKRMDFHFN | ubiquitination | [2] | | 518 | EKIEEIYKAFIKESG | ubiquitination | [1] | | 649 | PLSEKKMKAINSLGA | ubiquitination | [1] |
|
Reference | [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C. Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [ PMID: 21890473] [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity). |
Sequence Annotation | DOMAIN 275 373 SWIRM.
ZN_FING 133 193 CW-type.
NP_BIND 383 439 FAD (Potential).
MOD_RES 17 17 Phosphoserine.
MOD_RES 247 247 Phosphoserine. |
Keyword | 3D-structure; Alternative splicing; Chromatin regulator; Complete proteome; Developmental protein; FAD; Flavoprotein; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 822 AA |
Protein Sequence | MATPRGRTKK KASFDHSPDS LPLRSSGRQA KKKATETTDE DEDGGSEKKY RKCEKAGCTA 60
TCPVCFASAS ERCAKNGYTS RWYHLSCGEH FCNECFDHYY RSHKDGYDKY TTWKKIWTSN 120
GKTEPSPKAF MADQQLPYWV QCTKPECRKW RQLTKEIQLT PQIAKTYRCG MKPNTAIKPE 180
TSDHCSLPED LRVLEVSNHW WYSMLILPPL LKDSVAAPLL SAYYPDCVGM SPSCTSTNRA 240
AATGNASPGK LEHSKAALSV HVPGMNRYFQ PFYQPNECGK ALCVRPDVME LDELYEFPEY 300
SRDPTMYLAL RNLILALWYT NCKEALTPQK CIPHIIVRGL VRIRCVQEVE RILYFMTRKG 360
LINTGVLSVG ADQYLLPKDY HNKSVIIIGA GPAGLAAARQ LHNFGIKVTV LEAKDRIGGR 420
VWDDKSFKGV TVGRGAQIVN GCINNPVALM CEQLGISMHK FGERCDLIQE GGRITDPTID 480
KRMDFHFNAL LDVVSEWRKD KTQLQDVPLG EKIEEIYKAF IKESGIQFSE LEGQVLQFHL 540
SNLEYACGSN LHQVSARSWD HNEFFAQFAG DHTLLTPGYS VIIEKLAEGL DIQLKSPVQC 600
IDYSGDEVQV TTTDGTGYSA QKVLVTVPLA LLQKGAIQFN PPLSEKKMKA INSLGAGIIE 660
KIALQFPYRF WDSKVQGADF FGHVPPSASK RGLFAVFYDM DPQKKHSVLM SVIAGEAVAS 720
VRTLDDKQVL QQCMATLREL FKEQEVPDPT KYFVTRWSTD PWIQMAYSFV KTGGSGEAYD 780
IIAEDIQGTV FFAGEATNRH FPQTVTGAYL SGVREASKIA AF 822 |
Gene Ontology | GO:0005634; C:nucleus; ISS:UniProtKB. GO:0003677; F:DNA binding; IEA:InterPro. GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara. GO:0034648; F:histone demethylase activity (H3-dimethyl-K4 specific); ISS:UniProtKB. GO:0034649; F:histone demethylase activity (H3-monomethyl-K4 specific); ISS:UniProtKB. GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW. GO:0008270; F:zinc ion binding; IEA:Compara. GO:0043046; P:DNA methylation involved in gamete generation; IEA:Compara. GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW. GO:0044030; P:regulation of DNA methylation; ISS:UniProtKB. GO:0006349; P:regulation of gene expression by genetic imprinting; ISS:UniProtKB. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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