CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Butyrophilin subfamily 2 member A1 
Protein Synonyms/Alias
  
Gene Name
 BTN2A1 
Gene Synonyms/Alias
 BT2.1; BTF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
101GRTTFVSKDISRGSVubiquitination[1]
173ISRGWYPKPLTVWRDubiquitination[2]
230NTLLGQKKESVIFIPubiquitination[2]
295ETREIALKELEKERVubiquitination[2, 3, 4]
299IALKELEKERVQKEEubiquitination[2]
313EELQVKEKLQEELRWubiquitination[2]
444PDRILPLKESLCRVGubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 DOMAIN 29 141 Ig-like V-type.
 DOMAIN 310 506 B30.2/SPRY.
 CARBOHYD 46 46 N-linked (GlcNAc...) (Potential).
 CARBOHYD 114 114 N-linked (GlcNAc...).
 CARBOHYD 120 120 N-linked (GlcNAc...).
 DISULFID 51 125 By similarity.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MESAAALHFS RPASLLLLLL SLCALVSAQF IVVGPTDPIL ATVGENTTLR CHLSPEKNAE 60
DMEVRWFRSQ FSPAVFVYKG GRERTEEQME EYRGRTTFVS KDISRGSVAL VIHNITAQEN 120
GTYRCYFQEG RSYDEAILHL VVAGLGSKPL ISMRGHEDGG IRLECISRGW YPKPLTVWRD 180
PYGGVAPALK EVSMPDADGL FMVTTAVIIR DKSVRNMSCS INNTLLGQKK ESVIFIPESF 240
MPSVSPCAVA LPIIVVILMI PIAVCIYWIN KLQKEKKILS GEKEFERETR EIALKELEKE 300
RVQKEEELQV KEKLQEELRW RRTFLHAVDV VLDPDTAHPD LFLSEDRRSV RRCPFRHLGE 360
SVPDNPERFD SQPCVLGRES FASGKHYWEV EVENVIEWTV GVCRDSVERK GEVLLIPQNG 420
FWTLEMHKGQ YRAVSSPDRI LPLKESLCRV GVFLDYEAGD VSFYNMRDRS HIYTCPRSAF 480
SVPVRPFFRL GCEDSPIFIC PALTGANGVT VPEEGLTLHR VGTHQSL 527 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0006629; P:lipid metabolic process; TAS:ProtInc. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR013162; CD80_C2-set.
 IPR008985; ConA-like_lec_gl_sf.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR013106; Ig_V-set.
 IPR006574; PRY.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF08205; C2-set_2
 PF00622; SPRY
 PF07686; V-set 
SMART
 SM00409; IG
 SM00589; PRY
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50835; IG_LIKE 
PRINTS
 PR01407; BUTYPHLNCDUF.