CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013353
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GMP synthase [glutamine-hydrolyzing] 
Protein Synonyms/Alias
 GMP synthetase; Glutamine amidotransferase 
Gene Name
 Gmps 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
685IMYDLTSKPPGTTEWacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 DOMAIN 27 216 Glutamine amidotransferase type-1.
 DOMAIN 217 435 GMPS ATP-PPase.
 NP_BIND 244 250 ATP (By similarity).
 ACT_SITE 104 104 For GATase activity (By similarity).
 ACT_SITE 190 190 For GATase activity (By similarity).
 ACT_SITE 192 192 For GATase activity (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 9 9 N6-acetyllysine (By similarity).
 MOD_RES 318 318 Phosphothreonine (By similarity).
 MOD_RES 332 332 Phosphoserine (By similarity).  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Glutamine amidotransferase; GMP biosynthesis; Ligase; Nucleotide-binding; Phosphoprotein; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 693 AA 
Protein Sequence
MALCNGDSKP ENAGGDLKDG CHHYEGAVVI LDAGAQYGKV IDRRVRELFV QSEIFPLETP 60
AFAIKEQGFR AIIISGGPNS VYAEDAPWFD PAIFTIGKPV LGICYGMQMM NKVFGGTVHK 120
KSVREDGVFN ISMDNTCSLF RGLQKEEIVL LTHGDSVDKV ADGFKVVARS GNIVAGIANE 180
SKKLYGVQFH PEVGLTENGK VILKNFLYDI AGCSGNFTVQ NRELECIREI KEKVGTSKVL 240
VLLSGGVDST VCTALLNRAL NQDQVIAVHI DNGFMRKRES QSVEEALKKL GIQVKVINAA 300
HSFYNGTTTL PISDEDRTPR KRISKTLNMT TSPEEKRKII GDTFVKIANE VIGEMSLKPE 360
EVFLAQGTLR PDLIESASLV ASGKAELIKT HHNDTELIRK LREEGKVIEP LKDFHKDEVR 420
ILGRELDLPE ELVSRHPFPG PGLAIRVICA EEPYICKDFP ETNNILKIVA DFSASVKKPH 480
TLLQRVKACT TEEDQEKLME ITSQHSLNAF LLPIKTVGVQ GDCRSYSYVC GISSKDEPDW 540
ESLIFLARLI PRMCHNINRV VYIFGPPVKE PPTDVTPTFL TTGVLSTLRQ ADFEAHNILR 600
ESGYAGKISQ MPVILTPLHF DRDPLQKQPS CQRSVVIRTF ITSDFMTGVP ATPGNEIPVE 660
VVLKMVTEIK KIPGISRIMY DLTSKPPGTT EWE 693 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019899; F:enzyme binding; IDA:RGD.
 GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IDA:RGD.
 GO:0003921; F:GMP synthase activity; IDA:RGD.
 GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD. 
Interpro
 IPR017926; GATASE.
 IPR001674; GMP_synth_C.
 IPR004739; GMP_synth_N.
 IPR025777; GMPS_ATP_PPase_dom.
 IPR022310; NAD/GMP_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00117; GATase
 PF00958; GMP_synt_C
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1
 PS51553; GMPS_ATP_PPASE 
PRINTS