CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031165
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BRCA1-associated protein 
Protein Synonyms/Alias
 cDNA FLJ50182, highly similar to BRCA1-associated protein (EC 6.3.2.-) 
Gene Name
 BRAP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
202VHRLVASKTDGKIVQubiquitination[1, 2, 3, 4, 5, 6, 7]
206VASKTDGKIVQYECEubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
221GDTCQEEKIDALQLEubiquitination[2, 6, 7, 8]
247QRIYWENKIVRIEKDubiquitination[5]
263AEEINNMKTKFKETIubiquitination[5]
265EINNMKTKFKETIEKubiquitination[5]
267NNMKTKFKETIEKCDubiquitination[5]
328NQVLLQNKLKEEERVubiquitination[5]
401ASSGGSGKLPSRKGRubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 413 AA 
Protein Sequence
MHLYKTNKMT SLKEDVRRSA MLCILTVPAA MTSHDLMKFV APFNEVIEQM KIIRDSTPNQ 60
YMVLIKFRAQ GASLPVMDLT ELPKCTVCLE RMDESVNGIL TTLCNHSFHS QCLQRWDDTT 120
CPVCRYCQTP EPVEENKCFE CGVQENLWIC LICGHIGCGR YVSRHAYKHF EETQHTYAMQ 180
LTNHRVWDYA GDNYVHRLVA SKTDGKIVQY ECEGDTCQEE KIDALQLEYS YLLTSQLESQ 240
RIYWENKIVR IEKDTAEEIN NMKTKFKETI EKCDNLEHKL NDLLKEKQSV ERKCTQLNTK 300
VAKLTNELKE EQEMNKCLRA NQVLLQNKLK EEERVLKETC DQKDLQITEI QEQLRDVMFY 360
LETQQKINHL PAETRQEIQE GQINIAMASA SSPASSGGSG KLPSRKGRSK RGK 413 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR011422; BRAP2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR001607; Znf_UBP. 
Pfam
 PF07576; BRAP2
 PF13639; zf-RING_2
 PF02148; zf-UBP 
SMART
 SM00184; RING
 SM00290; ZnF_UBP 
PROSITE
 PS50089; ZF_RING_2
 PS50271; ZF_UBP 
PRINTS