UniProt Accession

 LUXS_ECOLI; P45578; P77134; P77805 

Genbank Protein ID

 U00096; AP009048; U83186; M86657 

Genbank Nucleotide ID

 AAC75734.1; BAA16549.1; AAB40286.1 

Protein Name

 S-ribosylhomocysteine lyase 

Protein Synonyms/Alias

 AI-2 synthesis protein; Autoinducer-2 production protein LuxS 

Gene Name

 luxS 

Gene Synonyms/Alias

 ygaG; b2687; JW2662 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
23	APAVRVAKTMNTPHG	acetylation	[1]
45	LRFCVPNKEVMPERG	acetylation	[1, 2]
105	QRVADAWKAAMEDVL	acetylation	[1]
163	NEELALPKEKLQELH	acetylation	[1]
163	NEELALPKEKLQELH	pupylation	[3]
165	ELALPKEKLQELHI*	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

 Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD). 

Sequence Annotation

 METAL 54 54 Iron (By similarity).
 METAL 58 58 Iron (By similarity).
 METAL 128 128 Iron (By similarity).  

Keyword

 Autoinducer synthesis; Complete proteome; Direct protein sequencing; Iron; Lyase; Metal-binding; Quorum sensing; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 171 AA 

Protein Sequence

Gene Ontology

 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0043768; F:S-ribosylhomocysteine lyase activity; IDA:EcoCyc.
 GO:0010699; P:cell-cell signaling involved in quorum sensing; IMP:EcoCyc.
 GO:0003001; P:generation of a signal involved in cell-cell signaling; IDA:EcoCyc.
 GO:0019284; P:L-methionine biosynthetic process from S-adenosylmethionine; IDA:EcoCyc. 

Interpro

 IPR011249; Metalloenz_LuxS/M16.
 IPR003815; S-ribosylhomocysteinase. 

Pfam

 PF02664; LuxS 

SMART

  

PROSITE

  

PRINTS

 PR01487; LUXSPROTEIN.