CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006608
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipase D1 
Protein Synonyms/Alias
 PLD 1; Choline phosphatase 1; Meiosis-specific sporulation-specific protein 14; Phosphatidylcholine-hydrolyzing phospholipase D1 
Gene Name
 SPO14 
Gene Synonyms/Alias
 PLD1; YKR031C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
1351ADATEQLKEWALNSLubiquitination[1]
1366ASKVLDDKEMIKSEIubiquitination[2]
1455NSLPLNEKSNEGESTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232] 
Functional Description
 Required for meiosis and spore formation. Seems to be involved in the coordinate induction of late meiotic events. PLD activity is induced under sporulation conditions and seems to be necessary to complete the meiotic cycle, but not for vegetative cell growth. 
Sequence Annotation
 DOMAIN 291 487 PX.
 DOMAIN 496 664 PH.
 DOMAIN 791 818 PLD phosphodiesterase 1.
 DOMAIN 1091 1118 PLD phosphodiesterase 2.
 ACT_SITE 796 796 Potential.
 ACT_SITE 798 798 Potential.
 ACT_SITE 803 803 Potential.
 ACT_SITE 1096 1096 Potential.
 ACT_SITE 1098 1098 Potential.
 ACT_SITE 1103 1103 Potential.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 30 30 Phosphoserine.
 MOD_RES 145 145 Phosphoserine.
 MOD_RES 1461 1461 Phosphoserine.
 MOD_RES 1462 1462 Phosphothreonine.  
Keyword
 Acetylation; Complete proteome; Hydrolase; Lipid degradation; Lipid metabolism; Meiosis; Phosphoprotein; Reference proteome; Repeat; Sporulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1683 AA 
Protein Sequence
MSNVSTASGT HFAPPQADRS VTEEVDRVNS RPDELENQEV LRQLPENGNL TSSLQREKRR 60
TPNGKEAERK HALPKSFVDR NLSDVSPNHS LDHIMHSNEH DPRRGSDEEN MHRLYNNLHS 120
SNNNVHSKRN SKREEERAPQ RRSSSVAYTQ QQFNGWKKEF GHAFKKISAI GRLKSSVNSP 180
TPAGSGHRHN QHQHQQVNEE DLYTQRLASD LLDSLLAGCP ASLFASTQFL RDEHGKRRAP 240
LLLAKLDVRV SPLKNDNNIL DITNSNHNHR GNNNNNTGEN SDRRPSIPRS SSIISISSNV 300
AEFMYSRNEN SLFRIHLEYG IDEDRLKWSI IRSYKDIKSL HHKLKIVAFQ QLTISKLYSD 360
NNRYHSLQLP HFPHYKEMVK ERNVMEKKAE NKPSSAASAP HTSENNNNDN GSNITSLETL 420
SSSEISEFNI DNVKMKHLQD LIDEPDDFSQ PIHLRLERYL RLLNIALCLR PHANRLFEFY 480
ELSPLGNLLS RESGFQGKQG YLVIRSTAKA QGWRVSHFGK HAFKDMIDRH TTKWFLVRNS 540
YLTYVSDLSS TTPLDVFLID WKFKVRFSGN KNNILDNENE INWIIHDPNL EINDELEEFG 600
IENDANNILD KNGKSKTHQK KSNISSKLLL LTLENSERKL KIICKSESSL KQWMSSIIKM 660
STSTPWSKPN RFGSFAPVRT NSFCKFLVDG RDYFWSLSEA LLMAKDVIYI HDWWLSPELY 720
LRRPVKGNQG FRIDRMLKSC AEKGIKIFIV IYRNVGNIVG TDSLWTKHSM LNLHPNIHII 780
RSPNQWLQNT YFWAHHEKFV VIDETFAFIG GTDLCYGRYD TFEHVLRDDA ESLLDQNFPG 840
KDYSNARIAD FHDLDKPFES MYDRKVIPRM PWHDVQMMTL GEPARDLARH FVQRWNYLLR 900
AKRPSRLTPL LTPPSDLTAE ELKSLPMFEI LREKSTCETQ ILRSAGNWSL GLKETECSIQ 960
NAYLKLIEQS EHFIYIENQF FITSTVWNGT CVLNKIGDAL VDRIVKANQE KKPWKAFILI 1020
PLMPGFDSPV DTAEASSLRL IMQFQYQSIS RGEHSTFSKL KKLNIDPAQY IQFFSLRKWS 1080
TFAPNERLIT EQLYVHAKIL IADDRRCIIG SANINERSQL GNRDSEVAIL IRDTDLIKTK 1140
MNGDDYYAGK FPWELRQRLM REHLGCDVDL VEFVEKKFER FEKFAAKNYE KLHTLSKEGD 1200
SGNNWSDREM IDSAMIELGY REIFGCKFSP QWKSGHGNSV DDGSTQCGIN EKEVGREDEN 1260
VYEKFFNSVD YGKSSRKRTP LPKHNFASLG LTFNHRAGIE NVGIRDHKVL STDPRLRKND 1320
EHKKEVDGYG PDCWKKESNK KFKADATEQL KEWALNSLAS KVLDDKEMIK SEIPEGFSNY 1380
LPNEKDLEMY LTDKTVTNRN KWSMLKRICY LQYLSHKLDE RKTQRLKKIK DMRRHLSSST 1440
ESTRNGSNSL PLNEKSNEGE STNVDQDIEG DEYHRLHEDI LKNQELDDGS LDDLLSQIIP 1500
KITNFNSGEI DDAKKEELLK LNFIDPYSFE DPLISSFSEG LWFTIALRNT LLYKLVFHCQ 1560
PDNAVQNWKE YGEFTELEQE FQINQEKLID LEAENINSTT TNVVDKDREK EKMRKAAELR 1620
MKLSGSLLYG FNQKVFDKHT AQRILERIHG HLVIFPTEWL AKEVESRNWI FNSDRLSPME 1680
IYN 1683 
Gene Ontology
 GO:0005768; C:endosome; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005628; C:prospore membrane; IDA:SGD.
 GO:0070290; F:NAPE-specific phospholipase D activity; IEA:EC.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0004630; F:phospholipase D activity; IDA:SGD.
 GO:0031321; P:ascospore-type prospore assembly; IMP:SGD.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0000753; P:cell morphogenesis involved in conjugation with cellular fusion; IMP:SGD.
 GO:0006887; P:exocytosis; IGI:SGD.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0006644; P:phospholipid metabolic process; IDA:SGD. 
Interpro
 IPR001683; Phox.
 IPR001849; Pleckstrin_homology.
 IPR001736; PLipase_D/transphosphatidylase.
 IPR016555; PLipase_D_euk.
 IPR015679; PLipase_D_fam. 
Pfam
 PF00614; PLDc 
SMART
 SM00233; PH
 SM00155; PLDc
 SM00312; PX 
PROSITE
 PS50003; PH_DOMAIN
 PS50035; PLD
 PS50195; PX 
PRINTS