CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022665
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma-associated antigen D1 
Protein Synonyms/Alias
 Dlxin-1; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homolog 
Gene Name
 Maged1 
Gene Synonyms/Alias
 Nrage 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
477ERANKLVKYLMLKDYubiquitination[1]
482LVKYLMLKDYTKVPIubiquitination[1]
486LMLKDYTKVPIKRSEubiquitination[1]
519RACFVLEKKFGIQLKubiquitination[1]
520ACFVLEKKFGIQLKEubiquitination[1]
602PLLGDLRKLLTYEFVubiquitination[1]
610LLTYEFVKQKYLDYRubiquitination[1]
612TYEFVKQKYLDYRRVubiquitination[1]
653RFIAEVQKRDPRDWTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds NGFR/p75NTR and antagonizes its association with NTRK1/TrkA, inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members (By similarity). May enhance ubiquitin ligase activity of RING-type zinc finger- containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 
Sequence Annotation
 REPEAT 292 297 1.
 REPEAT 298 303 2.
 REPEAT 304 309 3.
 REPEAT 329 334 4.
 REPEAT 335 340 5.
 REPEAT 341 346 6.
 REPEAT 347 352 7.
 REPEAT 353 358 8.
 REPEAT 359 364 9.
 REPEAT 365 370 10.
 REPEAT 371 376 11.
 REPEAT 377 382 12.
 REPEAT 383 388 13.
 REPEAT 389 394 14.
 REPEAT 395 400 15.
 REPEAT 401 406 16.
 REPEAT 407 412 17.
 REPEAT 413 418 18.
 REPEAT 419 424 19.
 REPEAT 425 429 20; approximate.
 REPEAT 430 435 21.
 REPEAT 436 441 22.
 DOMAIN 468 666 MAGE.
 REGION 292 441 22 X 6 AA tandem repeats of W-[PQ]-X-P-X-  
Keyword
 Cell membrane; Complete proteome; Cytoplasm; Membrane; Reference proteome; Repeat; Tumor antigen; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 775 AA 
Protein Sequence
MAQKPDGGAG LRGFQAEASV EDSALLVQTL MEAIQISEAP PTSQATAAAS GPNASPQSSQ 60
PPTANEKADT EVSAAAARPK TGFKAQNATT KGPNDYSQAR NAKEMPKNQS KAAFKSQNGT 120
PKGPHAASDF SQAAPTGKSA KKSEMAFKGQ NSTKAGPGTT YNFPQSPSAN EMTNNQPKTA 180
KAWNDTTKVP GADAQTQNVN QAKMADVGTS AGISEADGAA AQTSADGSQT QNVESRTIIR 240
GKRTRKVNNL NVEENNSGDQ RRASLASGNW RSAPVPVTTQ QNPPGAPPNV VWQTPLAWQN 300
PSGWQNQTAR QTPPAARQSP PARQTPSAWQ NPVAWQNPVI WPNPVIWQNP VIWPNPIVWP 360
GPIVWPNPMA WQSTPGWQSP PSWQAPPSWQ SPQDWQGPPD WQVPPDWSMP PDWSFPSDWP 420
FPPDWIPADW PIPPDWQNLR PSPNLRSSSN SRASQNQGPP QPRDVALLQE RANKLVKYLM 480
LKDYTKVPIK RSEMLRDIIR EYTDVYPEII ERACFVLEKK FGIQLKEIDK EEHLYILIST 540
PESLAGILGT TKDTPKLGLL LVILGIIFMN GNRATEAVLW EALRKMGLRP GVRHPLLGDL 600
RKLLTYEFVK QKYLDYRRVP NSNPPEYEFL WGLRSYHETS KMKVLRFIAE VQKRDPRDWT 660
AQFMEAADEA LDALDAAAAE AEARAEARNR MGIGDEAVSG PWSWDDIEFE LLTWDEEGDF 720
GDPWSRIPFT FWARYHQNAR SRFPQAFTGP IIGPSGTATA NFAANFGAIG FFWVE 775 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; IDA:MGI.
 GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
 GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; IMP:UniProtKB.
 GO:0043406; P:positive regulation of MAP kinase activity; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI. 
Interpro
 IPR002190; MAGE. 
Pfam
 PF01454; MAGE 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS