| Tag | Content |
|---|
| CPLM ID | CPLM-011100 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Pre-mRNA-splicing factor CEF1 |
Protein Synonyms/Alias | PRP nineteen-associated complex protein 85; PRP19-associated complex protein 85 |
Gene Name | CEF1 |
Gene Synonyms/Alias | NTC85; YMR213W; YM8261.07 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 240 | EDRQIKKKFEQFERK | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Involved in pre-mRNA splicing and cell cycle control. Required for the binding of the NTC complex (or PRP19-associated complex) components to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Its absence leads to an arrest of the cell cycle, possibly due to the inefficient splicing of TUB1. |
Sequence Annotation | DOMAIN 1 60 HTH myb-type 1.
DOMAIN 63 110 HTH myb-type 2.
DNA_BIND 33 56 H-T-H motif (By similarity).
DNA_BIND 84 106 H-T-H motif (By similarity).
REGION 460 490 Interaction with PRP19 and self- |
Keyword | Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 590 AA |
Protein Sequence | MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL 60
NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE RYNRLLESED SGGAALSTGV 120
TDLKAGDINP NAETQMARPD NGDLEDEEKE MLAEARARLL NTQGKKATRK IRERMLEESK 180
RIAELQKRRE LKQAGINVAI KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK 240
FEQFERKVNR KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS 300
APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS NIKSGKQLKS 360
RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK EFENERAMNE EDNFIEPPSQ 420
NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA IDNKYNLLVA NAINKEPHMV PEDTVDFLKE 480
VESRMQHITQ GRTSMKIQFK TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM 540
CSTLCHHSLP ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI 590 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0000974; C:Prp19 complex; IDA:SGD. GO:0071006; C:U2-type catalytic step 1 spliceosome; IDA:SGD. GO:0003682; F:chromatin binding; IEA:InterPro. GO:0003677; F:DNA binding; IEA:UniProtKB-KW. GO:0007049; P:cell cycle; IEA:UniProtKB-KW. GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |