CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020299
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein LSM14 homolog B 
Protein Synonyms/Alias
 Protein FAM61B; RNA-associated protein 55B; hRAP55B 
Gene Name
 LSM14B 
Gene Synonyms/Alias
 C20orf40; FAM61B; RAP55B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13GTPYLGSKISLISKAubiquitination[1, 2]
19SKISLISKAQIRYEGubiquitination[2]
42NSTVALAKVRSFGTEubiquitination[2, 3]
172SADNLNAKKLLPGKGubiquitination[4]
314GPNCYYDKSKSFFDNubiquitination[2]
316NCYYDKSKSFFDNISubiquitination[2, 5]
327DNISSELKTSSRRTTubiquitination[5, 6]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May play a role in control of mRNA translation (By similarity). 
Sequence Annotation
 DOMAIN 241 277 DFDF.
 MOTIF 310 326 FFD box.
 MOTIF 330 350 TFG box.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 115 115 Phosphoserine.
 MOD_RES 154 154 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Developmental protein; Phosphoprotein; Reference proteome; Ribonucleoprotein; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 385 AA 
Protein Sequence
MSGSSGTPYL GSKISLISKA QIRYEGILYT IDTDNSTVAL AKVRSFGTED RPTDRPAPPR 60
EEIYEYIIFR GSDIKDITVC EPPKAQHTLP QDPAIVQSSL GSASASPFQP HVPYSPFRGM 120
APYGPLAASS LLSQQYAASL GLGAGFPSIP VGKSPMVEQA VQTGSADNLN AKKLLPGKGT 180
TGTQLNGRQA QPSSKTASDV VQPAAVQAQG QVNDENRRPQ RRRSGNRRTR NRSRGQNRPT 240
NVKENTIKFE GDFDFESANA QFNREELDKE FKKKLNFKDD KAEKGEEKDL AVVTQSAEAP 300
AEEDLLGPNC YYDKSKSFFD NISSELKTSS RRTTWAEERK LNTETFGVSG RFLRGRSSRG 360
GFRGGRGNGT TRRNPTSHRA GTGRV 385 
Gene Ontology
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 
Interpro
 IPR025762; DFDF.
 IPR019050; FDF_dom.
 IPR025761; FFD_box.
 IPR025609; Lsm14_N.
 IPR010920; LSM_dom.
 IPR025768; TFG_box. 
Pfam
 PF09532; FDF
 PF12701; LSM14 
SMART
  
PROSITE
 PS51512; DFDF
 PS51513; FFD
 PS51536; TFG 
PRINTS