CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018413
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid oxidation complex subunit alpha 
Protein Synonyms/Alias
 Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
 fadJ 
Gene Synonyms/Alias
 STM2388 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
478ATTVKLAKKQGKTPIacetylation[1]
479TTVKLAKKQGKTPIVacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By similarity). 
Sequence Annotation
 REGION 1 190 Enoyl-CoA hydratase (By similarity).
 REGION 306 714 3-hydroxyacyl-CoA dehydrogenase (By  
Keyword
 Complete proteome; Cytoplasm; Fatty acid metabolism; Isomerase; Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 715 AA 
Protein Sequence
MTTTSAFMLN VRLDNVAVVA IDVPGEKVNT LKAEFATQVR AILKQIRENK ALQGVVFISA 60
KADNFIAGAD INMIGHCQNA QEAETLARQG QQLMAEIQAL PVPVIAAIHG ACLGGGLEMA 120
LACHRRICTD DVKTVLGLPE VQLGLLPGSG GTQRLPRLVG VSTALDMILT GKQLRARQAL 180
KAGLVDDVVP QTILLEAAVE LAKKERLAQR TLPVRERILA GPLGRALLFR LVRKKTAQKT 240
QGNYPATERI IDVIETGLAQ GSSSGYDAEA RAFGELAMTP QSQALRAIFF ASTEVKKDPG 300
SDAPPGPLNS VGILGGGLMG GGIAWVTACK GGLPVRIKDI NTQGINHALK YSWDLLETKV 360
RRRHIKASER DKQLALISGS TDYRGFSHRD LVIEAVFEDL PLKQQMVAEV EQNCAAHTIF 420
ASNTSSLPIG DIAANAARPE QVIGLHFFSP VEKMPLVEVI PHASTSAQTI ATTVKLAKKQ 480
GKTPIVVSDK AGFYVNRILA PYINEAIRML TEGERVEHID AALVKFGFPV GPIQLLDEVG 540
IDTGTKIIPV LEAAYGERFS APANVVASIL NDDRKGRKNG RGFYLYGEKG RKSKKQVDPA 600
IYKLIGVQGQ SRLSAQQVAE RCVMLMLNEA ARCFDEKVIR SARDGDIGAV FGIGFPPFLG 660
GPFRYMDALG PGEMVATLQR LAALYGPRYA PCEQLVRMAE RREHFWTNGE TDQGN 715 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
 GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
 GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR001753; Crotonase_core_superfam.
 IPR013328; DH_multihelical.
 IPR012802; FadJ.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00725; 3HCDH
 PF02737; 3HCDH_N
 PF00378; ECH 
SMART
  
PROSITE
 PS00067; 3HCDH
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS