UniProt Accession

 HINT1_MOUSE; P70349 

Genbank Protein ID

 U60001; AK002965; AK012433; BC070415; BC080296 

Genbank Nucleotide ID

 AAC71076.1; BAB22484.1; BAB28235.1; AAH70415.1; AAH80296.1 

Protein Name

 Histidine triad nucleotide-binding protein 1 

Protein Synonyms/Alias

 Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1 

Gene Name

 Hint1 

Gene Synonyms/Alias

 Hint; Pkci; Pkci1; Prkcnh1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
30	IRKEIPAKIIFEDDR	acetylation	[1]
83	HLMIVGKKCAADLGL	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex (By similarity). 

Sequence Annotation

 DOMAIN 18 126 HIT.
 NP_BIND 43 44 Purine nucleotide phosphoramidate (By
 NP_BIND 105 107 Purine nucleotide phosphoramidate (By
 NP_BIND 112 114 Purine nucleotide phosphoramidate (By
 MOTIF 110 114 Histidine triad motif.
 ACT_SITE 112 112 Tele-AMP-histidine intermediate (By
 BINDING 99 99 Purine nucleotide phosphoramidate (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 21 21 N6-acetyllysine (By similarity).
 MOD_RES 30 30 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 126 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000118; C:histone deacetylase complex; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0016787; F:hydrolase activity; ISS:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; ISS:UniProtKB.
 GO:0009154; P:purine ribonucleotide catabolic process; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR019808; Histidine_triad_CS.
 IPR001310; Histidine_triad_HIT.
 IPR011146; HIT-like. 

Pfam

 PF01230; HIT 

SMART

  

PROSITE

 PS00892; HIT_1
 PS51084; HIT_2 

PRINTS

 PR00332; HISTRIAD.