CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014668
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trifunctional purine biosynthetic protein adenosine-3 
Protein Synonyms/Alias
 Phosphoribosylamine--glycine ligase; Glycinamide ribonucleotide synthetase; GARS; Phosphoribosylglycinamide synthetase; Phosphoribosylformylglycinamidine cyclo-ligase; AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase; Phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART 
Gene Name
 Gart 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
285MLTKDGPKVLEFNCRubiquitination[1]
438RPRGLTYKDSGVDIAubiquitination[1]
452AAGNMLVKKIQPLAKubiquitination[1]
459KKIQPLAKATSRPGCubiquitination[1]
485DLKAAGFKDPLLASGacetylation[2]
485DLKAAGFKDPLLASGubiquitination[1]
697IPRVLPQKFGVDLDAubiquitination[1]
866PTRVINHKLYKNRVEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
  
Sequence Annotation
 DOMAIN 111 318 ATP-grasp.
 NP_BIND 137 199 ATP (By similarity).
 REGION 434 807 AIRS.
 REGION 808 1010 GART.
 REGION 818 820 5'-phosphoribosylglycinamide binding (By
 REGION 896 899 10-formyltetrahydrofolate binding (By
 REGION 947 951 10-formyltetrahydrofolate binding (By
 REGION 977 980 5'-phosphoribosylglycinamide binding (By
 ACT_SITE 915 915 Proton donor (By similarity).
 METAL 288 288 Manganese (By similarity).
 METAL 290 290 Manganese (By similarity).
 BINDING 871 871 10-formyltetrahydrofolate (By
 BINDING 913 913 10-formyltetrahydrofolate (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 350 350 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Ligase; Manganese; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Purine biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1010 AA 
Protein Sequence
MAARVLVIGS GGREHTLAWK LAQSPQVKQV LVAPGNAGTA CAGKISNAAV SVNDHSALAQ 60
FCKDEKIELV VVGPEAPLAA GIVGDLTSAG VRCFGPTAQA AQLESSKKFA KEFMDRHEIP 120
TAQWRAFTNP EDACSFITSA NFPALVVKAS GLAAGKGVIV AKSQAEACRA VQEIMQEKSF 180
GAAGETVVVE EFLEGEEVSC LCFTDGKTVA EMPPAQDHKR LLDGDEGPNT GGMGAYCPAP 240
QVSKDLLVKI KNTILQRAVD GMQQEGAPYT GILYAGIMLT KDGPKVLEFN CRFGDPECQV 300
ILPLLKSDLY EVMQSTLDGL LSASLPVWLE NHSAVTVVMA SKGYPGAYTK GVEITGFPEA 360
QALGLQVFHA GTALKDGKVV TSGGRVLTVT AVQENLMSAL AEARKGLAAL KFEGAIYRKD 420
IGFRAVAFLQ RPRGLTYKDS GVDIAAGNML VKKIQPLAKA TSRPGCSVDL GGFAGLFDLK 480
AAGFKDPLLA SGTDGVGTKL KIAQLCNKHD SIGQDLVAMC VNDILAQGAE PLFFLDYFSC 540
GKLDLSTTEA VIAGIAAACQ QAGCALLGGE TAEMPNMYPP GEYDLAGFAV GAMERHQKLP 600
QLERITEGDA VIGVASSGLH SNGFSLVRKI VERSSLQYSS PAPGGCGDQT LGDLLLTPTR 660
IYSHSLLPII RSGRVKAFAH ITGGGLLENI PRVLPQKFGV DLDASTWRVP KVFSWLQQEG 720
ELSEEEMART FNCGIGAALV VSKDQAEQVL HDVRRRQEEA WVIGSVVACP EDSPRVRVKN 780
LIETIQTNGS LVANGFLKSN FPVQQKKARV AVLISGTGSN LQALIDSTRD PKSSSHIVLV 840
ISNKAAVAGL DRAERAGIPT RVINHKLYKN RVEFDNAVDH VLEEFSVDIV CLAGFMRILS 900
GPFVRKWDGK MLNIHPSLLP SFKGSNAHEQ VLEAGVTITG CTVHFVAEDV DAGQIILQEA 960
VPVRRGDTVA TLSERVKVAE HKIFPAALQL VASGAVQLRE DGKIHWAKEQ 1010 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008168; F:methyltransferase activity; IEA:InterPro.
 GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:EC.
 GO:0004641; F:phosphoribosylformylglycinamidine cyclo-ligase activity; IEA:EC.
 GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IEA:EC.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0032259; P:methylation; IEA:GOC.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro. 
Interpro
 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR002376; Formyl_transf_N.
 IPR001555; GART_AS.
 IPR016185; PreATP-grasp_dom.
 IPR020561; PRibGlycinamid_synth_ATP-grasp.
 IPR000115; PRibGlycinamide_synth.
 IPR020560; PRibGlycinamide_synth_C-dom.
 IPR020559; PRibGlycinamide_synth_CS.
 IPR020562; PRibGlycinamide_synth_N.
 IPR004733; PurM_cligase.
 IPR016188; PurM_N-like.
 IPR004607; PurN_trans.
 IPR011054; Rudment_hybrid_motif. 
Pfam
 PF00586; AIRS
 PF02769; AIRS_C
 PF00551; Formyl_trans_N
 PF01071; GARS_A
 PF02843; GARS_C
 PF02844; GARS_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP
 PS00184; GARS
 PS00373; GART 
PRINTS