CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007566
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Epidermal growth factor receptor substrate 15 
Protein Synonyms/Alias
 Protein Eps15; Protein AF-1p 
Gene Name
 EPS15 
Gene Synonyms/Alias
 AF1P 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
479PGKRSINKLDSPDPFubiquitination[1]
487LDSPDPFKLNDPFQPubiquitination[1]
502FPGNDSPKEKDPEIFubiquitination[1]
504GNDSPKEKDPEIFCDubiquitination[1]
522SATTTTNKEADPSNFubiquitination[1]
546EDMIEWAKRESEREEubiquitination[1]
576ELAIALSKSEISEA*ubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta- 1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. 
Sequence Annotation
 DOMAIN 15 104 EH 1.
 DOMAIN 128 216 EH 2.
 DOMAIN 160 195 EF-hand 1.
 DOMAIN 223 258 EF-hand 2.
 DOMAIN 224 314 EH 3.
 REPEAT 599 601 1.
 REPEAT 623 625 2.
 REPEAT 629 631 3.
 REPEAT 634 636 4.
 REPEAT 640 642 5.
 REPEAT 645 647 6.
 REPEAT 651 653 7.
 REPEAT 664 666 8.
 REPEAT 672 674 9.
 REPEAT 692 694 10.
 REPEAT 709 711 11.
 REPEAT 737 739 12.
 REPEAT 798 800 13.
 REPEAT 804 806 14.
 REPEAT 825 827 15.
 REPEAT 851 870 UIM 1.
 REPEAT 877 896 UIM 2.
 REGION 2 330 Interaction with DAB2 (By similarity).
 REGION 599 827 15 X 3 AA repeats of D-P-F.
 MOTIF 768 774 SH3-binding.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 108 108 Phosphoserine.
 MOD_RES 140 140 Phosphoserine.
 MOD_RES 323 323 Phosphoserine.
 MOD_RES 324 324 Phosphoserine.
 MOD_RES 467 467 Phosphoserine.
 MOD_RES 470 470 Phosphoserine.
 MOD_RES 485 485 Phosphoserine.
 MOD_RES 562 562 Phosphoserine (By similarity).
 MOD_RES 563 563 Phosphoserine (By similarity).
 MOD_RES 777 777 Phosphothreonine (By similarity).
 MOD_RES 779 779 Phosphothreonine (By similarity).
 MOD_RES 790 790 Phosphoserine.
 MOD_RES 796 796 Phosphoserine.
 MOD_RES 814 814 Phosphoserine.
 MOD_RES 849 849 Phosphotyrosine; by EGFR.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Calcium; Cell membrane; Chromosomal rearrangement; Coated pit; Complete proteome; Cytoplasm; Endocytosis; Endosome; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Protein transport; Proto-oncogene; Reference proteome; Repeat; SH3-binding; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 896 AA 
Protein Sequence
MYLKSDSGLG GWITIPAVAD VLKYSCIVCW SSREKNNVEQ DLKEKEDTIK QRTSEVQDLQ 60
DEVQRENTNL QKLQAQKQQV QELLDELDEQ KAQLEEQLKE VRKKCAEEAQ LISSLKAELT 120
SQESQISTYE EELAKAREEL SRLQQETAEL EESVESGKAQ LEPLQQHLQD SQQEISSMQM 180
KLMEMKDLEN HNSQLNWCSS PHSILVNGAT DYCSLSTSSS ETANLNEHVE GQSNLESEPI 240
HQESPARSSP ELLPSGVTDE NEVTTAVTEK VCSELDNNRH SKEEDPFNVD SSSLTGPVAD 300
TNLDFFQSDP FVGSDPFKDD PFGKIDPFGG DPFKGSDPFA SDCFFRQSTD PFATSSTDPF 360
SAANNSSITS VETLKHNDPF APGGTVVAAS DSATDPFASV FGNESFGGGF ADFSTLSKVN 420
NEDPFRSATS SSVSNVVITK NVFEETSVKS EDEPPALPPK IGTPTRPCPL PPGKRSINKL 480
DSPDPFKLND PFQPFPGNDS PKEKDPEIFC DPFTSATTTT NKEADPSNFA NFSAYPSEED 540
MIEWAKRESE REEEQRLARL NQQEQEDLEL AIALSKSEIS EA 582 
Gene Ontology
 GO:0030122; C:AP-2 adaptor complex; IEA:Compara.
 GO:0060170; C:cilium membrane; IEA:Compara.
 GO:0005905; C:coated pit; TAS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HGNC.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0048268; P:clathrin coat assembly; IDA:BHF-UCL.
 GO:0032456; P:endocytic recycling; IC:BHF-UCL.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR003903; Ubiquitin-int_motif. 
Pfam
  
SMART
 SM00054; EFh
 SM00027; EH
 SM00726; UIM 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50330; UIM 
PRINTS