| Tag | Content |
|---|
| CPLM ID | CPLM-007321 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Vacuolar protein sorting-associated protein 27 |
Protein Synonyms/Alias | Golgi retention defective protein 11 |
Gene Name | VPS27 |
Gene Synonyms/Alias | DID7; GRD11; SSV17; VPL23; VPT27; YNR006W; N2038 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 147 | KLISRGIKFPEKLTL | ubiquitination | [1] | | 151 | RGIKFPEKLTLSNSP | ubiquitination | [1] | | 232 | CFEDYDLKRHDDSKK | ubiquitination | [1] | | 272 | KAIELSLKESRNSAS | ubiquitination | [1] | | 290 | IVPVVESKNEVKRQE | ubiquitination | [1] | | 294 | VESKNEVKRQEIEEE | ubiquitination | [2] | | 321 | LREAEEAKLRSERQK | ubiquitination | [1] | | 403 | LNYALNDKAQKYNTL | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat. |
Sequence Annotation | DOMAIN 18 149 VHS.
REPEAT 258 277 UIM 1.
REPEAT 301 320 UIM 2.
ZN_FING 170 230 FYVE-type.
MOD_RES 157 157 Phosphoserine.
MOD_RES 495 495 Phosphoserine.
MOD_RES 613 613 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Endosome; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 622 AA |
Protein Sequence | MSVSTPSELD ALIEQATSES IPNGDLDLPI ALEISDVLRS RRVNPKDSMR CIKKRILNTA 60
DNPNTQLSSW KLTNICVKNG GTPFIKEICS REFMDTMEHV ILREDSNEEL SELVKTILYE 120
LYVAFKNDSQ LNYVAKVYDK LISRGIKFPE KLTLSNSPTA MFDSKTPADW IDSDACMICS 180
KKFSLLNRKH HCRSCGGVFC QEHSSNSIPL PDLGIYEPVR VCDSCFEDYD LKRHDDSKKS 240
KKHRHKRKKD RDYSTPEDEE ELIRKAIELS LKESRNSASS EPIVPVVESK NEVKRQEIEE 300
EEDPDLKAAI QESLREAEEA KLRSERQKAS RQMQPQQPSP QPQPIHSVDL SDEEKDSIYM 360
FASLVEKMKS RPLNEILEDS KLQNLAQRVF ASKARLNYAL NDKAQKYNTL IEMNGKISEI 420
MNIYDRLLEQ QLQSINLSQQ YTLPQVPSDP YNYLTENVQN PAESYQTPPL QQLSSHQYKP 480
QQDVSRQQSV KANSSPTTNI DHLKTIDVTP HAQQKPQSHV ELAPSDPPYP KEEAEDEGTQ 540
AVQDEESSTQ ESRERPYPVE TENGETSINK RPQGITRYDF PTVPARKFVQ PESTVPLPAS 600
SSEIPIKEER PPSPQEELLI EL 622 |
Gene Ontology | GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell. GO:0033565; C:ESCRT-0 complex; IPI:SGD. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD. GO:0043130; F:ubiquitin binding; IDA:SGD. GO:0045324; P:late endosome to vacuole transport; IMP:SGD. GO:0045053; P:protein retention in Golgi apparatus; IMP:SGD. GO:0006623; P:protein targeting to vacuole; IMP:SGD. |
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Pfam | |
SMART | |
PROSITE | |
PRINTS | |