CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006368
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4B 
Protein Synonyms/Alias
 eIF-4B 
Gene Name
 TIF3 
Gene Synonyms/Alias
 STM1; TIF42; YPR163C; P9325.6 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
155GNAFVTLKERADLVAacetylation[1]
376KTTDEQPKIQKSVYDubiquitination[2]
379DEQPKIQKSVYDVLRacetylation[1]
398DEDEEAEKQNGDAKEubiquitination[2]
414KVDAAVEKLQDKTAQacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in translation initiation. May be the homolog of mammalian eIF4B and be part of a RNA helicase. STM1/TIF3 is a non-essential gene. 
Sequence Annotation
 DOMAIN 101 183 RRM.
 REPEAT 190 210 1; approximate.
 REPEAT 211 232 2.
 REPEAT 233 258 3.
 REPEAT 259 284 4.
 REPEAT 285 310 5.
 REPEAT 311 340 6.
 REPEAT 341 350 7; truncated.
 REGION 190 350 7 X approximate tandem repeats.
 MOD_RES 65 65 Phosphoserine.
 MOD_RES 71 71 Phosphoserine.  
Keyword
 Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 436 AA 
Protein Sequence
MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL SELAHAKNNS 60
NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP YRAVINNIPW DITPEGVQAW 120
VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA FVTLKERADL VAVLKFNGTK LNERTVYVSV 180
AAPRRGGGAD VDWSSARGSN FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR 240
GSNFQGSSRP PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS 300
RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF GKPQQTKNTY 360
KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN GDAKENKVDA AVEKLQDKTA 420
QLTVEDGDNW EVVGKK 436 
Gene Ontology
 GO:0005840; C:ribosome; TAS:SGD.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0033592; F:RNA strand annealing activity; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; ISS:SGD.
 GO:0002181; P:cytoplasmic translation; IMP:SGD. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
  
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS