CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000180
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Circadian locomoter output cycles protein kaput 
Protein Synonyms/Alias
 mCLOCK 
Gene Name
 Clock 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
186SLTPEYLKSKNQLEFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 ARNTL/2-CLOCK heterodimers activate E-box element (5'- CACGTG-3') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: its own partner, ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. 
Sequence Annotation
 DOMAIN 34 84 bHLH.
 DOMAIN 107 177 PAS 1.
 DOMAIN 262 332 PAS 2.
 DOMAIN 336 379 PAC.
 REGION 514 564 Implicated in the circadian rhythmicity.
 MOD_RES 408 408 Phosphoserine.  
Keyword
 3D-structure; Activator; Acyltransferase; Alternative splicing; Biological rhythms; Chromosome; Complete proteome; Cytoplasm; DNA damage; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 855 AA 
Protein Sequence
MVFTVSCSKM SSIVDRDDSS IFDGLVEEDD KDKAKRVSRN KSEKKRRDQF NVLIKELGSM 60
LPGNARKMDK STVLQKSIDF LRKHKETTAQ SDASEIRQDW KPTFLSNEEF TQLMLEALDG 120
FFLAIMTDGS IIYVSESVTS LLEHLPSDLV DQSIFNFIPE GEHSEVYKIL STHLLESDSL 180
TPEYLKSKNQ LEFCCHMLRG TIDPKEPSTY EYVRFIGNFK SLTSVSTSTH NGFEGTIQRT 240
HRPSYEDRVC FVATVRLATP QFIKEMCTVE EPNEEFTSRH SLEWKFLFLD HRAPPIIGYL 300
PFEVLGTSGY DYYHVDDLEN LAKCHEHLMQ YGKGKSCYYR FLTKGQQWIW LQTHYYITYH 360
QWNSRPEFIV CTHTVVSYAE VRAERRRELG IEESLPETAA DKSQDSGSDN RINTVSLKEA 420
LERFDHSPTP SASSRSSRKS SHTAVSDPSS TPTKIPTDTS TPPRQHLPAH EKMTQRRSSF 480
SSQSINSQSV GPSLTQPAMS QAANLPIPQG MSQFQFSAQL GAMQHLKDQL EQRTRMIEAN 540
IHRQQEELRK IQEQLQMVHG QGLQMFLQQS NPGLNFGSVQ LSSGNSNIQQ LTPVNMQGQV 600
VPANQVQSGH ISTGQHMIQQ QTLQSTSTQQ SQQSVMSGHS QQTSLPSQTP STLTAPLYNT 660
MVISQPAAGS MVQIPSSMPQ NSTQSATVTT FTQDRQIRFS QGQQLVTKLV TAPVACGAVM 720
VPSTMLMGQV VTAYPTFATQ QQQAQTLSVT QQQQQQQQQP PQQQQQQQQS SQEQQLPSVQ 780
QPAQAQLGQP PQQFLQTSRL LHGNPSTQLI LSAAFPLQQS TFPPSHHQQH QPQQQQQLPR 840
HRTDSLTDPS KVQPQ 855 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IDA:UniProtKB.
 GO:0070888; F:E-box binding; IEA:Compara.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IC:BHF-UCL.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IDA:BHF-UCL.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0008134; F:transcription factor binding; ISA:MGI.
 GO:0071479; P:cellular response to ionizing radiation; IEA:Compara.
 GO:0032922; P:circadian regulation of gene expression; IMP:UniProtKB.
 GO:0000077; P:DNA damage checkpoint; IEA:Compara.
 GO:0016573; P:histone acetylation; IEA:GOC. 
Interpro
 IPR011598; bHLH_dom.
 IPR001067; Nuc_translocat.
 IPR001610; PAC.
 IPR000014; PAS.
 IPR013767; PAS_fold.
 IPR013655; PAS_fold_3. 
Pfam
 PF00010; HLH
 PF00989; PAS
 PF08447; PAS_3 
SMART
 SM00353; HLH
 SM00086; PAC
 SM00091; PAS 
PROSITE
 PS50888; BHLH
 PS50113; PAC
 PS50112; PAS 
PRINTS
 PR00785; NCTRNSLOCATR.