| Tag | Content |
|---|
| CPLM ID | CPLM-039184 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | tRNA (guanine(37)-N1)-methyltransferase |
Protein Synonyms/Alias | M1G-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5 homolog |
Gene Name | Trmt5 |
Gene Synonyms/Alias | Trm5; Trmt5_predicted; rCG_61480 |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 289 | KLNKVDQKVKVFNMD | acetylation | [1] |
|
Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding (By similarity). |
Sequence Annotation | REGION 268 269 S-adenosyl-L-methionine binding (By
REGION 296 297 S-adenosyl-L-methionine binding (By
BINDING 230 230 S-adenosyl-L-methionine (By similarity).
BINDING 328 328 S-adenosyl-L-methionine (By similarity). |
Keyword | Complete proteome; Cytoplasm; Methyltransferase; Mitochondrion; Nucleus; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 449 AA |
Protein Sequence | MPEAEASVRD RELFSPPSDV RGMTKLDRTA FKKTVPIPVL KARKEVVSRL MRALRRVALQ 60
RPGIKRVIED PKDEDSRLIM LDPYRMLTSD SFEKPELSAL KELDVNPQLS QYNLELTYEN 120
FKSEEILRAV LPEGQDVTSG FSRVGHIAHL NLRDHQLPFK HLIGQVMVDK NPGITSAVNK 180
TSNIDNTYRN FQMEVLCGEE NMLTKVRENN YTYEFDFSKV YWNPRLSTEH GRITELLNPG 240
DVLFDVFAGV GPFAIPAARK NCTVFANDLN PESHRWLLHN CKLNKVDQKV KVFNMDGKDF 300
LQGPVREELM LRLGLSTEGK PSLHIVMNLP AKAIEFLSVF RSLLDGQPCS TERLPIVHCY 360
CFSKDSDPAK DARQRAEAVL GVCLEASSSV HLVRNVAPNK EMLCITFQIP TATLYRDQAL 420
GLQNDQEPPL KRQKTGEPFS GEPQSASDS 449 |
Gene Ontology | GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0052906; F:tRNA (guanine(37)-N(1))-methyltransferase activity; IEA:EC. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |