UniProt Accession

 4F2_HUMAN; P08195; Q13543 

Genbank Protein ID

 J02939; J02769; J03569; M21904; M21898; M21899; M21900; M21901; M21902; M21903; AB018010; AP001160; BC001061; BC003000; BE794697 

Genbank Nucleotide ID

 AAA52497.1; AAA51540.1; AAA35536.1; AAA35489.1; AAA35489.1; AAA35489.1; AAA35489.1; AAA35489.1; AAA35489.1; AAA35489.1; BAA84649.1; AAH01061.2; AAH03000.2 

Protein Name

 4F2 cell-surface antigen heavy chain 

Protein Synonyms/Alias

 4F2hc; 4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; CD98 

Gene Name

 SLC3A2 

Gene Synonyms/Alias

 MDU1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
171	MSLAGAEKNGLVKIK	ubiquitination	[1]
178	KNGLVKIKVAEDEAE	ubiquitination	[2, 3]
191	AEAAAAAKFTGLSKE	ubiquitination	[1, 3]
197	AKFTGLSKEELLKVA	ubiquitination	[1, 3]
248	CRELPAQKWWHTGAL	ubiquitination	[3]
276	AGNLAGLKGRLDYLS	ubiquitination	[1, 3]
286	LDYLSSLKVKGLVLG	ubiquitination	[3]
288	YLSSLKVKGLVLGPI	ubiquitination	[3]
300	GPIHKNQKDDVAQTD	ubiquitination	[3]
362	DTVATKVKDALEFWL	ubiquitination	[3]
385	VRDIENLKDASSFLA	ubiquitination	[3]
646	PLELERLKLEPHEGL	ubiquitination	[1, 3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 103 103 Phosphoserine.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 406 406 Phosphoserine (Probable).
 MOD_RES 408 408 Phosphoserine (Probable).
 MOD_RES 410 410 Phosphoserine (Probable).
 MOD_RES 527 527 Phosphoserine (Probable).
 MOD_RES 531 531 Phosphoserine (Probable).
 CARBOHYD 365 365 N-linked (GlcNAc...).
 CARBOHYD 381 381 N-linked (GlcNAc...).
 CARBOHYD 424 424 N-linked (GlcNAc...).
 CARBOHYD 506 506 N-linked (GlcNAc...).
 DISULFID 210 210 Interchain (with light chain).
 CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Alternative splicing; Amino-acid transport; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 630 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
 GO:0009986; C:cell surface; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005432; F:calcium:sodium antiporter activity; TAS:UniProtKB.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0015175; F:neutral amino acid transmembrane transporter activity; ISS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
 GO:0016049; P:cell growth; NAS:UniProtKB.
 GO:0060356; P:leucine import; ISS:UniProtKB.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0015827; P:tryptophan transport; ISS:UniProtKB. 

Interpro

 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 

Pfam

 PF00128; Alpha-amylase 

SMART

  

PROSITE

  

PRINTS