CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021508
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain-containing protein 9 
Protein Synonyms/Alias
 Rhabdomyosarcoma antigen MU-RMS-40.8 
Gene Name
 BRD9 
Gene Synonyms/Alias
 UNQ3040/PRO9856 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26YADKPLEKPLKLVLKacetylation[1]
26YADKPLEKPLKLVLKubiquitination[1, 2]
155FLRQLQRKDPHGFFAubiquitination[2]
322NRFLPGGKMGYLKRNubiquitination[1, 2]
373GFTTLGFKDERRNKVacetylation[3, 4]
373GFTTLGFKDERRNKVubiquitination[1, 2]
438DAGSYSKKVVDDLLDubiquitination[2]
460SRTLFQLKQRRNVPMubiquitination[2]
512LSSLGKVKKELDPDDubiquitination[1, 2]
513SSLGKVKKELDPDDSubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 May play a role in chromatin remodeling and regulation of transcription. 
Sequence Annotation
 DOMAIN 153 223 Bromo.
 MOD_RES 373 373 N6-acetyllysine.
 MOD_RES 566 566 Phosphoserine.
 MOD_RES 588 588 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Bromodomain; Chromatin regulator; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MGKKHKKHKA EWRSSYEDYA DKPLEKPLKL VLKVGGSEVT ELSGSGHDSS YYDDRSDHER 60
ERHKEKKKKK KKKSEKEKHL DDEERRKRKE EKKRKREREH CDTEGEADDF DPGKKVEVEP 120
PPDRPVRACR TQPAENESTP IQQLLEHFLR QLQRKDPHGF FAFPVTDAIA PGYSMIIKHP 180
MDFGTMKDKI VANEYKSVTE FKADFKLMCD NAMTYNRPDT VYYKLAKKIL HAGFKMMSKQ 240
AALLGNEDTA VEEPVPEVVP VQVETAKKSK KPSREVISCM FEPEGNACSL TDSTAEEHVL 300
ALVEHAADEA RDRINRFLPG GKMGYLKRNG DGSLLYSVVN TAEPDADEEE THPVDLSSLS 360
SKLLPGFTTL GFKDERRNKV TFLSSATTAL SMQNNSVFGD LKSDEMELLY SAYGDETGVQ 420
CALSLQEFVK DAGSYSKKVV DDLLDQITGG DHSRTLFQLK QRRNVPMKPP DEAKVGDTLG 480
DSSSSVLEFM SMKSYPDVSV DISMLSSLGK VKKELDPDDS HLNLDETTKL LQDLHEAQAE 540
RGGSRPSSNL SSLSNASERD QHHLGSPSRL SVGEQPDVTH DPYEFLQSPE PAASAKT 597 
Gene Ontology
 GO:0070577; F:histone acetyl-lysine binding; IDA:UniProtKB.
 GO:0003676; F:nucleic acid binding; NAS:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain.
 IPR021900; DUF3512. 
Pfam
 PF00439; Bromodomain
 PF12024; DUF3512 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2 
PRINTS
 PR00503; BROMODOMAIN.