UniProt Accession

 NKX25_MOUSE; P42582; P97335 

Genbank Protein ID

 L20300; AF091351; X75415 

Genbank Nucleotide ID

 AAC97934.1; CAA53169.1 

Protein Name

 Homeobox protein Nkx-2.5 

Protein Synonyms/Alias

 Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E 

Gene Name

 Nkx2-5 

Gene Synonyms/Alias

 Csx; Nkx-2.5; Nkx2e 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
51	SCMLAAFKPEAYSGP	sumoylation	[1, 2, 3]

Reference

 [1] Regulation of cardiac specific nkx2.5 gene activity by small ubiquitin-like modifier.
 Wang J, Zhang H, Iyer D, Feng XH, Schwartz RJ.
 J Biol Chem. 2008 Aug 22;283(34):23235-43. [PMID: 18579533]
 [2] Expression of sumoylation deficient Nkx2.5 mutant in Nkx2.5 haploinsufficient mice leads to congenital heart defects.
 Kim EY, Chen L, Ma Y, Yu W, Chang J, Moskowitz IP, Wang J.
 PLoS One. 2011;6(6):e20803. [PMID: 21677783]
 [3] Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5.
 Costa MW, Lee S, Furtado MB, Xin L, Sparrow DB, Martinez CG, Dunwoodie SL, Kurtenbach E, Mohun T, Rosenthal N, Harvey RP.
 PLoS One. 2011;6(9):e24812. [PMID: 21931855] 

Functional Description

 Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression. 

Sequence Annotation

 DNA_BIND 137 196 Homeobox.  

Keyword

 Complete proteome; Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 318 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IMP:BHF-UCL.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IMP:BHF-UCL.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0010736; F:serum response element binding; IDA:BHF-UCL.
 GO:0007512; P:adult heart development; IMP:MGI.
 GO:0003278; P:apoptotic process involved in heart morphogenesis; IMP:MGI.
 GO:0055014; P:atrial cardiac muscle cell development; TAS:BHF-UCL.
 GO:0060413; P:atrial septum morphogenesis; IEA:Compara.
 GO:0060928; P:atrioventricular node cell development; IMP:MGI.
 GO:0060929; P:atrioventricular node cell fate commitment; IMP:MGI.
 GO:0030509; P:BMP signaling pathway; IDA:MGI.
 GO:0003166; P:bundle of His development; IGI:MGI.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IDA:MGI.
 GO:0060038; P:cardiac muscle cell proliferation; IGI:MGI.
 GO:0060048; P:cardiac muscle contraction; IMP:MGI.
 GO:0003211; P:cardiac ventricle formation; IGI:MGI.
 GO:0060971; P:embryonic heart tube left/right pattern formation; IMP:MGI.
 GO:0007492; P:endoderm development; TAS:BHF-UCL.
 GO:0001947; P:heart looping; IMP:MGI.
 GO:0060347; P:heart trabecula formation; IMP:MGI.
 GO:0030097; P:hemopoiesis; IMP:MGI.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IDA:BHF-UCL.
 GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IMP:BHF-UCL.
 GO:0010832; P:negative regulation of myotube differentiation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0003151; P:outflow tract morphogenesis; IMP:MGI.
 GO:0003148; P:outflow tract septum morphogenesis; IEA:Compara.
 GO:0060037; P:pharyngeal system development; IGI:MGI.
 GO:0051891; P:positive regulation of cardioblast differentiation; IMP:BHF-UCL.
 GO:0008284; P:positive regulation of cell proliferation; IGI:MGI.
 GO:0045823; P:positive regulation of heart contraction; IMP:BHF-UCL.
 GO:0045666; P:positive regulation of neuron differentiation; IEA:Compara.
 GO:0010765; P:positive regulation of sodium ion transport; IMP:BHF-UCL.
 GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0010735; P:positive regulation of transcription via serum response element binding; IDA:BHF-UCL.
 GO:1901387; P:positive regulation of voltage-gated calcium channel activity; IMP:BHF-UCL.
 GO:0003342; P:proepicardium development; IMP:MGI.
 GO:0003350; P:pulmonary myocardium development; IMP:MGI.
 GO:0003168; P:Purkinje myocyte differentiation; IMP:MGI.
 GO:0060043; P:regulation of cardiac muscle cell proliferation; IDA:MGI.
 GO:0055117; P:regulation of cardiac muscle contraction; IMP:BHF-UCL.
 GO:0003221; P:right ventricular cardiac muscle tissue morphogenesis; IEA:Compara.
 GO:0045214; P:sarcomere organization; IGI:MGI.
 GO:0003285; P:septum secundum development; IEA:Compara.
 GO:0048536; P:spleen development; TAS:BHF-UCL.
 GO:0030878; P:thyroid gland development; IMP:BHF-UCL.
 GO:0043586; P:tongue development; TAS:BHF-UCL.
 GO:0001570; P:vasculogenesis; IMP:MGI.
 GO:0055005; P:ventricular cardiac myofibril development; IMP:MGI.
 GO:0060412; P:ventricular septum morphogenesis; IEA:Compara.
 GO:0003222; P:ventricular trabecula myocardium morphogenesis; IMP:MGI. 

Interpro

 IPR017970; Homeobox_CS.
 IPR020479; Homeobox_metazoa.
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like. 

Pfam

 PF00046; Homeobox 

SMART

 SM00389; HOX 

PROSITE

 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2 

PRINTS

 PR00024; HOMEOBOX.