CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014280
UniProt Accession
Genbank Protein ID
 U34883 
Genbank Nucleotide ID
Protein Name
 Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 
Protein Synonyms/Alias
 PAPS synthase 1; PAPSS 1; Sulfurylase kinase 1; SK 1; SK1; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase 
Gene Name
 Papss1 
Gene Synonyms/Alias
 Asapk; Atpsk1; Papss 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
12GSLCKKVKLSNNAQNacetylation[1]
183KARAGEIKGFTGIDSubiquitination[2]
404RLTPTELKQKFKDMNubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. 
Sequence Annotation
 NP_BIND 59 66 ATP (Potential).
 REGION 1 ?220 Adenylyl-sulfate kinase.
 REGION ?221 624 Sulfate adenylyltransferase.
 MOTIF 521 525 PP-motif.
 ACT_SITE 133 133 Phosphoserine intermediate (By  
Keyword
 ATP-binding; Complete proteome; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 624 AA 
Protein Sequence
MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF RGCTVWLTGL 60
SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG FSPEDREENV RRIAEVAKLF 120
ADAGLVCITS FISPYTQDRN NARQIHEGAS LPFFEVFVDA PLHVCEQRDV KGLYKKARAG 180
EIKGFTGIDS EYEKPEAPEL VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP 240
ENKLHLAKTD AEALPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG 300
VINLSVPIVL TATHEDKERL DGCTAFALVY EGRRVAILRN PEFFEHRKEE RCARQWGTTC 360
KNHPYIKMVL EQGDWLIGGD LQVLDRIYWN DGLDQYRLTP TELKQKFKDM NADAVFAFQL 420
RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL LHPLGGWTKD DDVPLMWRMK QHAAVLEEGI 480
LDPETTVVAI FPSPMMYAGP TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPT 540
HGAKVLTMAP GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK 600
PPEGFMAPKA WTVLVEYYKS LEKA 624 
Gene Ontology
 GO:0009336; C:sulfate adenylyltransferase complex (ATP); IC:MGI.
 GO:0004020; F:adenylylsulfate kinase activity; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004781; F:sulfate adenylyltransferase (ATP) activity; ISS:UniProtKB.
 GO:0050428; P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; IDA:MGI.
 GO:0000103; P:sulfate assimilation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR002891; APS_kinase.
 IPR025980; ATP-Sase_PUA-like_dom.
 IPR027417; P-loop_NTPase.
 IPR015947; PUA-like_domain.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR024951; Sulfurylase_cat_dom.
 IPR002650; Sulphate_adenylyltransferase. 
Pfam
 PF01583; APS_kinase
 PF01747; ATP-sulfurylase
 PF14306; PUA_2 
SMART
  
PROSITE
  
PRINTS