UniProt Accession

 AIFM1_RAT; Q9JM53; Q548E3 

Genbank Protein ID

 AB041723; AF375656; BC072697 

Genbank Nucleotide ID

 BAA94745.1; AAM46094.1; AAH72697.1 

Protein Name

 Apoptosis-inducing factor 1, mitochondrial 

Protein Synonyms/Alias

 Programmed cell death protein 8 

Gene Name

 Aifm1 

Gene Synonyms/Alias

 Aif; Pdcd8 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
198	QFRQWNGKERSIYFQ	acetylation	[1]
231	VAVLTGKKVVHLDVR	acetylation	[1]
243	DVRGNMVKLNDGSQI	acetylation	[1]
336	VIQLFPEKGNMGKIL	acetylation	[1]
341	PEKGNMGKILPEYLS	acetylation	[1]
387	IKLKDGRKVETDHIV	acetylation	[1]
589	NRMPIARKIIKDGEQ	acetylation	[1]
592	PIARKIIKDGEQHED	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity). 

Sequence Annotation

 NP_BIND 137 141 FAD (By similarity).
 NP_BIND 163 164 FAD (By similarity).
 NP_BIND 453 454 FAD (By similarity).
 REGION 133 482 FAD-dependent oxidoreductase (By
 MOTIF 445 450 Nuclear localization signal (Potential).
 BINDING 171 171 FAD (By similarity).
 BINDING 176 176 FAD (By similarity).
 BINDING 232 232 FAD; via amide nitrogen and carbonyl
 BINDING 284 284 FAD (By similarity).
 BINDING 437 437 FAD (By similarity).
 BINDING 482 482 FAD; via carbonyl oxygen (By similarity).
 MOD_RES 267 267 Phosphoserine (By similarity).
 MOD_RES 592 592 N6-acetyllysine (By similarity).
 CROSSLNK 254 254 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; FAD; Flavoprotein; Isopeptide bond; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 612 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005758; C:mitochondrial intermembrane space; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; ISS:UniProtKB.
 GO:0006309; P:apoptotic DNA fragmentation; IEA:Compara.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; ISS:UniProtKB.
 GO:0032981; P:mitochondrial respiratory chain complex I assembly; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0030182; P:neuron differentiation; IEA:Compara. 

Interpro

 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 

Pfam

 PF00070; Pyr_redox
 PF07992; Pyr_redox_2 

SMART

  

PROSITE

  

PRINTS

 PR00368; FADPNR.