CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020994
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit F 
Protein Synonyms/Alias
 eIF3f; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47 
Gene Name
 Eif3f 
Gene Synonyms/Alias
 Eif3s5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
242MFTPLTVKYAYYDTEacetylation[1]
242MFTPLTVKYAYYDTEubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 
Sequence Annotation
 DOMAIN 91 200 MPN.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 52 52 Phosphoserine; by CDK11; in vitro (By
 MOD_RES 242 242 N6-acetyllysine (By similarity).
 MOD_RES 262 262 Phosphoserine (By similarity).  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Initiation factor; Phosphoprotein; Protease; Protein biosynthesis; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MASPAVPANV PPATAAAAPA PVVTAAPASA PTPSTPAPTP AATPAASPAP VSSDPAVAAP 60
AAPGQTPASA PAPAQTPAPS QPGPALPGPF PGGRVVRLHP VILASIVDSY ERRNEGAARV 120
IGTLLGTVDK HSVEVTNCFS VPHNESEDEV AVDMEFAKNM YELHKKVSPN ELILGWYATG 180
HDITEHSVLI HEYYSREAPN PIHLTVDTGL QHGRMSIKAY VSTLMGVPGR TMGVMFTPLT 240
VKYAYYDTER IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG 300
KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS QIALNEKLVN 360
L 361 
Gene Ontology
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 
Interpro
 IPR027531; eIF3f.
 IPR000555; JAB1_Mov34_MPN_PAD1.
 IPR024969; Rpn11/EIF3F_C. 
Pfam
 PF01398; JAB
 PF13012; MitMem_reg 
SMART
 SM00232; JAB_MPN 
PROSITE
  
PRINTS