CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023080
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box only protein 3 
Protein Synonyms/Alias
  
Gene Name
 FBXO3 
Gene Synonyms/Alias
 FBX3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59LWRRHCKKYWLISEEubiquitination[1]
119PRMVLSLKEGAREEDubiquitination[1, 2, 3, 4]
153YRIHNGQKLVVPGLLubiquitination[1]
218EAAEGRNKNEVFYQCubiquitination[1]
322SKDALPEKACQLDSRubiquitination[5]
337YWRITNAKGDVEEVQubiquitination[1, 3, 6]
390HFLYFKDKIFNVAIPubiquitination[2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Substrate recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53- dependent transactivation. 
Sequence Annotation
 DOMAIN 10 56 F-box.
 DOMAIN 278 408 ApaG.  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MAAMETETAP LTLESLPTDP LLLILSFLDY RDLINCCYVS RRLSQLSSHD PLWRRHCKKY 60
WLISEEEKTQ KNQCWKSLFI DTYSDVGRYI DHYAAIKKAW DDLKKYLEPR CPRMVLSLKE 120
GAREEDLDAV EAQIGCKLPD DYRCSYRIHN GQKLVVPGLL GSMALSNHYR SEDLLDVDTA 180
AGGFQQRQGL KYCLPLTFCI HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI 240
IGATFTDWFT SYVKNVVSGG FPIIRDQIFR YVHDPECVAT TGDITVSVST SFLPELSSVH 300
PPHYFFTYRI RIEMSKDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG EFPIISPGRV 360
YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK IFNVAIPRFH MACPTFRVSI ARLEMGPDEY 420
EEMEEEEEEE EEEDEDDDSA DMDESDEDDE EERRRRVFDV PIRRRRCSRL F 471 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
 GO:0006508; P:proteolysis; TAS:ProtInc. 
Interpro
 IPR007474; ApaG_domain.
 IPR018958; Cell_wall_assmbl_KNR4-like.
 IPR001810; F-box_dom_cyclin-like. 
Pfam
 PF04379; DUF525
 PF00646; F-box 
SMART
 SM00256; FBOX
 SM00860; SMI1_KNR4 
PROSITE
 PS51087; APAG
 PS50181; FBOX 
PRINTS