UniProt Accession

 NUOG_ECOLI; P33602; P76489; P78184; P78185 

Genbank Protein ID

 X68301; U00096; AP009048; L25055 

Genbank Nucleotide ID

 CAA48366.1; AAC75343.2; BAA16111.2; AAA03538.1 

Protein Name

 NADH-quinone oxidoreductase subunit G 

Protein Synonyms/Alias

 NADH dehydrogenase I subunit G; NDH-1 subunit G; NUO7 

Gene Name

 nuoG 

Gene Synonyms/Alias

 b2283; JW2278 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
276	GYGYVNLKDRPRQPV	acetylation	[1]
642	DPAYYDSKTVMLESW	acetylation	[1]
775	FQDEVGGKLRFGDPG	acetylation	[1]
809	RFQPQDGKWRIAPYY	acetylation	[1]
849	LNPADAAKLGVNAGT	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. 

Sequence Annotation

 DOMAIN 2 83 2Fe-2S ferredoxin-type.
 DOMAIN 221 277 4Fe-4S Mo/W bis-MGD-type.
 METAL 34 34 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 45 45 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 48 48 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 67 67 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 99 99 Iron-sulfur 2 (4Fe-4S); via pros nitrogen
 METAL 103 103 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 106 106 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 112 112 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 151 151 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 154 154 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 157 157 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 201 201 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 228 228 Iron-sulfur 4 (4Fe-4S) (By similarity).
 METAL 231 231 Iron-sulfur 4 (4Fe-4S) (By similarity).
 METAL 235 235 Iron-sulfur 4 (4Fe-4S) (By similarity).
 METAL 263 263 Iron-sulfur 4 (4Fe-4S) (By similarity).  

Keyword

 2Fe-2S; 4Fe-4S; Cell inner membrane; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; NAD; Oxidoreductase; Quinone; Reference proteome; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 908 AA 

Protein Sequence

Gene Ontology

 GO:0045272; C:plasma membrane respiratory chain complex I; IDA:EcoCyc.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; IDA:EcoCyc.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0030151; F:molybdenum ion binding; IEA:InterPro.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:RefGenome.
 GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
 GO:0009060; P:aerobic respiration; IMP:EcoCyc.
 GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro. 

Interpro

 IPR001041; 2Fe-2S_ferredoxin-type.
 IPR009010; Asp_de-COase-like_dom.
 IPR012675; Beta-grasp_dom.
 IPR006657; MoPterin_dinucl-bd_dom.
 IPR006656; Mopterin_OxRdtase.
 IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
 IPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS.
 IPR010228; NADH_UbQ_OxRdtase_Gsu.
 IPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. 

Pfam

 PF04879; Molybdop_Fe4S4
 PF00384; Molybdopterin
 PF01568; Molydop_binding
 PF10588; NADH-G_4Fe-4S_3 

SMART

 SM00926; Molybdop_Fe4S4
 SM00929; NADH-G_4Fe-4S_3 

PROSITE

 PS00197; 2FE2S_FER_1
 PS51085; 2FE2S_FER_2
 PS51669; 4FE4S_MOW_BIS_MGD
 PS00641; COMPLEX1_75K_1
 PS00642; COMPLEX1_75K_2
 PS00643; COMPLEX1_75K_3 

PRINTS