UniProt Accession

 H2B_NEUCR; P37210; Q7S3Y6; Q8X131 

Genbank Protein ID

 AY062171; AABX02000010 

Genbank Nucleotide ID

 AAL38971.1; EAA30204.1 

Protein Name

 Histone H2B 

Protein Synonyms/Alias

  

Gene Name

 hh2b 

Gene Synonyms/Alias

 NCU02435 

Created Date

 July 27, 2013 

Organism

 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DS 

NCBI Taxa ID

 367110 

Lysine Modification

Position	Peptide	Type	References
8	MPPKPADKKPASKAP	acetylation	[1]
9	PPKPADKKPASKAPA	acetylation	[1, 2]
13	ADKKPASKAPATASK	acetylation	[1, 2]
20	KAPATASKAPEKKDA	acetylation	[1, 2]
29	PEKKDAGKKTAASGD	acetylation	[1, 2]
30	EKKDAGKKTAASGDK	acetylation	[2]
54	TYSSYIYKVLKQVHP	acetylation	[1]
57	SYIYKVLKQVHPDTG	acetylation	[1]
90	RVATEASKLAAYNKK	acetylation	[1]
96	SKLAAYNKKSTISSR	acetylation	[1]
97	KLAAYNKKSTISSRE	acetylation	[1]
119	ILPGELAKHAVSEGT	acetylation	[1]

Reference

 [1] Extensive and varied modifications in histone H2B of wild-type and histone deacetylase 1 mutant Neurospora crassa.
 Anderson DC, Green GR, Smith K, Selker EU.
 Biochemistry. 2010 Jun 29;49(25):5244-57. [PMID: 20462202]
 [2] Mapping of lysine methylation and acetylation in core histones of Neurospora crassa.
 Xiong L, Adhvaryu KK, Selker EU, Wang Y.
 Biochemistry. 2010 Jun 29;49(25):5236-43. [PMID: 20433192] 

Functional Description

 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. 

Sequence Annotation

 MOD_RES 8 8 N6-acetyllysine; alternate (By
 MOD_RES 9 9 N6-acetyllysine; alternate (By
 MOD_RES 12 12 Phosphoserine (By similarity).
 MOD_RES 13 13 N6-acetyllysine (By similarity).
 MOD_RES 24 24 N6-acetyllysine; alternate (By
 CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 9 9 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 24 24 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 25 25 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 131 131 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 137 AA 

Protein Sequence

Gene Ontology

 GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR000558; Histone_H2B. 

Pfam

 PF00125; Histone 

SMART

 SM00427; H2B 

PROSITE

 PS00357; HISTONE_H2B 

PRINTS

 PR00621; HISTONEH2B.