UniProt Accession

 K2C8_RAT; Q10758; Q5WPB3 

Genbank Protein ID

 M63482; M63482; S76054; AY464139; BC091106; BC097497 

Genbank Nucleotide ID

 AAA19667.1; AAA19668.1; AAR36875.1; AAH91106.1; AAH97497.1 

Protein Name

 Keratin, type II cytoskeletal 8 

Protein Synonyms/Alias

 Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 

Gene Name

 Krt8 

Gene Synonyms/Alias

 Krt2-8 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
122	QNKMLETKWSLLQQQ	acetylation	[1]
158	LEALGQEKLKLEVEL	acetylation	[1]
198	NEFVLIKKDVDEAYM	acetylation	[1]
207	VDEAYMNKVELESRL	acetylation	[1]
285	AETMYQIKYEELQTL	acetylation	[1]
295	ELQTLAGKHGDDLRR	acetylation	[1]
304	GDDLRRSKTEISEMN	acetylation	[1]
325	QAEIDALKGQRATLE	acetylation	[1]
347	QRGELAVKDANAKLE	acetylation	[1]
352	AVKDANAKLEDLKNA	acetylation	[1]
357	NAKLEDLKNALQKAK	acetylation	[1]
464	RTKAVVVKKIETRDG	acetylation	[1]
472	KIETRDGKLVSESSD	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. 

Sequence Annotation

 REGION 1 90 Head.
 REGION 91 398 Rod.
 REGION 91 126 Coil 1A.
 REGION 127 143 Linker 1.
 REGION 144 235 Coil 1B.
 REGION 236 259 Linker 12.
 REGION 260 398 Coil 2.
 REGION 261 382 Necessary for interaction with PNN (By
 REGION 399 483 Tail.
 MOD_RES 9 9 Phosphoserine; by PKC/PRKCE (Probable).
 MOD_RES 13 13 Phosphoserine.
 MOD_RES 21 21 Phosphoserine (By similarity).
 MOD_RES 24 24 Phosphoserine; by PKC/PRKCE (Probable).
 MOD_RES 27 27 Phosphoserine (By similarity).
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 36 36 Phosphoserine (By similarity).
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 43 43 Phosphoserine.
 MOD_RES 46 46 Phosphoserine (By similarity).
 MOD_RES 47 47 Phosphoserine (By similarity).
 MOD_RES 51 51 Phosphoserine.
 MOD_RES 101 101 N6-malonyllysine (By similarity).
 MOD_RES 117 117 N6-acetyllysine (By similarity).
 MOD_RES 207 207 N6-acetyllysine (By similarity).
 MOD_RES 253 253 Phosphoserine (By similarity).
 MOD_RES 258 258 Phosphoserine (By similarity).
 MOD_RES 295 295 N6-acetyllysine (By similarity).
 MOD_RES 325 325 N6-acetyllysine (By similarity).
 MOD_RES 347 347 N6-acetyllysine (By similarity).
 MOD_RES 400 400 Phosphoserine (By similarity).
 MOD_RES 410 410 Phosphoserine (By similarity).
 MOD_RES 417 417 Phosphoserine.
 MOD_RES 424 424 Phosphoserine.
 MOD_RES 426 426 Phosphoserine.
 MOD_RES 432 432 Phosphoserine (By similarity).
 MOD_RES 475 475 Phosphoserine (By similarity).
 MOD_RES 478 478 Phosphoserine (By similarity).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Glycoprotein; Intermediate filament; Keratin; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0043034; C:costamere; IDA:RGD.
 GO:0016010; C:dystrophin-associated glycoprotein complex; IDA:UniProtKB.
 GO:0045095; C:keratin filament; IDA:RGD.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0042383; C:sarcolemma; IDA:UniProtKB.
 GO:0030018; C:Z disc; IDA:UniProtKB.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:Compara.
 GO:0060706; P:cell differentiation involved in embryonic placenta development; IEA:Compara.
 GO:0051599; P:response to hydrostatic pressure; IDA:RGD.
 GO:0051707; P:response to other organism; IEA:Compara.
 GO:0045214; P:sarcomere organization; IDA:UniProtKB.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:Compara. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR018039; Intermediate_filament_CS.
 IPR003054; Keratin_II. 

Pfam

 PF00038; Filament 

SMART

  

PROSITE

 PS00226; IF 

PRINTS

 PR01276; TYPE2KERATIN.