UniProt Accession

 DOM3Z_YEAST; P53063; D6VV89 

Genbank Protein ID

 X94357; Z72768; AY693165; BK006941 

Genbank Nucleotide ID

 CAA64141.1; CAA96966.1; AAT93184.1; DAA07873.1 

Protein Name

 Decapping nuclease RAI1 

Protein Synonyms/Alias

 RAT1-interacting protein 

Gene Name

 RAI1 

Gene Synonyms/Alias

 YGL246C; NRE387 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
281	PRIIYGFKDDHYVLK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping and pyrophosphohydrolase activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates. Stimulates exoribonuclease activity of RAT1, allowing it to degrade RNAs with stable secondary structure more effectively. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II. 

Sequence Annotation

 REGION 273 387 Interaction with RAT1.
 METAL 172 172 Divalent metal cation (By similarity).
 METAL 223 223 Divalent metal cation (By similarity).
 METAL 241 241 Divalent metal cation (By similarity).
 METAL 242 242 Divalent metal cation; via carbonyl
 BINDING 105 105 Substrate (By similarity).
 BINDING 221 221 Substrate (By similarity).
 BINDING 267 267 Substrate (By similarity).
 MOD_RES 198 198 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; mRNA processing; Nuclease; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; rRNA processing; Transcription; Transcription regulation; Transcription termination. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 387 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0030234; F:enzyme regulator activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0016462; F:pyrophosphatase activity; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0000956; P:nuclear-transcribed mRNA catabolic process; IMP:SGD.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IMP:SGD. 

Interpro

 IPR013961; RAI1. 

Pfam

 PF08652; RAI1 

SMART

  

PROSITE

  

PRINTS