UniProt Accession

 IFIT3_MOUSE; Q64345; Q99L62 

Genbank Protein ID

 U43086; L32974; BC003804 

Genbank Nucleotide ID

 AAC52627.1; AAA39329.1; AAH03804.1 

Protein Name

 Interferon-induced protein with tetratricopeptide repeats 3 

Protein Synonyms/Alias

 IFIT-3; Glucocorticoid-attenuated response gene 49 protein; GARG-49; P49; IRG2 

Gene Name

 Ifit3 

Gene Synonyms/Alias

 Garg49; Ifi49; Isg49 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
246	NQTDVLQKAAQFYKK	ubiquitination	[1]
320	LTMEFMQKTLQRRRS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2 (By similarity). 

Sequence Annotation

 REPEAT 94 127 TPR 1.
 REPEAT 136 169 TPR 2.
 REPEAT 172 206 TPR 3.
 REPEAT 241 274 TPR 4.  

Keyword

 Antiviral defense; Complete proteome; Cytoplasm; Immunity; Innate immunity; Mitochondrion; Reference proteome; Repeat; TPR repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 403 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0035457; P:cellular response to interferon-alpha; IEA:InterPro.
 GO:0035458; P:cellular response to interferon-beta; IDA:MGI.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:0035634; P:response to stilbenoid; IEP:UniProtKB.
 GO:0009615; P:response to virus; ISS:UniProtKB. 

Interpro

 IPR024122; Interferon-induced_IFIT3.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 

Pfam

 PF00515; TPR_1
 PF07719; TPR_2
 PF13181; TPR_8 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS