UniProt Accession

 RL8A_YEAST; P17076; D3DKT5 

Genbank Protein ID

 X17204; X56836; U17361; U11583; BK006934 

Genbank Nucleotide ID

 CAA35073.1; CAA40166.1; AAA64574.1; AAB65045.1; DAA06652.1 

Protein Name

 60S ribosomal protein L8-A 

Protein Synonyms/Alias

 L4; L4-2; L7a-1; Maintenance of killer protein 7; RP6; YL5 

Gene Name

 RPL8A 

Gene Synonyms/Alias

 MAK7; RPL4A; YHL033C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
6	**MAPGKKVAPAPFG	ubiquitination	[1]
15	APAPFGAKSTKSNKT	acetylation	[2]
32	PLTHSTPKNFGIGQA	acetylation	[3]
43	IGQAVQPKRNLSRYV	acetylation	[3]
51	RNLSRYVKWPEYVRV	acetylation	[3]
51	RNLSRYVKWPEYVRV	ubiquitination	[1]
70	KILSIRLKVPPTIAQ	ubiquitination	[1]
92	NTAAETFKLFNKYRP	acetylation	[3]
92	NTAAETFKLFNKYRP	ubiquitination	[1]
96	ETFKLFNKYRPETAA	acetylation	[3]
111	EKKERLTKEAAAVAE	acetylation	[3]
111	EKKERLTKEAAAVAE	ubiquitination	[1]
120	AAAVAEGKSKQDASP	ubiquitination	[1]
122	AVAEGKSKQDASPKP	ubiquitination	[1]
128	SKQDASPKPYAVKYG	acetylation	[3]
172	FLPALCKKMGVPYAI	ubiquitination	[1]
181	GVPYAIVKGKARLGT	acetylation	[3]
193	LGTLVNQKTSAVAAL	ubiquitination	[1]
213	EDEAALAKLVSTIDA	ubiquitination	[4]
225	IDANFADKYDEVKKH	acetylation	[3]
230	ADKYDEVKKHWGGGI	acetylation	[3]
231	DKYDEVKKHWGGGIL	acetylation	[3]
241	GGGILGNKAQAKMDK	acetylation	[3]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 256 AA 

Protein Sequence

Gene Ontology

 GO:0022625; C:cytosolic large ribosomal subunit; TAS:SGD.
 GO:0003735; F:structural constituent of ribosome; TAS:SGD.
 GO:0002181; P:cytoplasmic translation; TAS:SGD.
 GO:0042254; P:ribosome biogenesis; IEA:InterPro. 

Interpro

 IPR001921; Ribosomal_L7A/L8.
 IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
 IPR018492; Ribosomal_L7Ae/L8/Nhp2.
 IPR004037; Ribosomal_L7Ae_CS. 

Pfam

 PF01248; Ribosomal_L7Ae 

SMART

  

PROSITE

 PS01082; RIBOSOMAL_L7AE 

PRINTS

 PR00881; L7ARS6FAMILY.
 PR00882; RIBOSOMALL7A.