CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019503
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin repeat domain-containing protein 27 
Protein Synonyms/Alias
 VPS9 domain-containing protein 
Gene Name
 ANKRD27 
Gene Synonyms/Alias
 PP12899 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10LYDEDLLKNPFYLALubiquitination[1]
136PSDPFSLKTIEDVREubiquitination[1]
196LLRDSHLKMLAKQEAubiquitination[2]
200SHLKMLAKQEAQMNLubiquitination[2]
252SLQDLQQKDIGVKPEubiquitination[1]
257QQKDIGVKPEFSFNIubiquitination[1]
276RELAQLNKCTSPQQKubiquitination[1]
411HIASGNQKEVERLLSubiquitination[1, 2]
429HDKDTVQKMCHPLCFubiquitination[1]
442CFCDDCEKLVSGRLNubiquitination[1]
626LSFERRQKSSEAPVQmethylation[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 May be a Rab21 guanine exchange factor and regulate endosome dynamics (By similarity). 
Sequence Annotation
 DOMAIN 233 371 VPS9.
 REPEAT 396 426 ANK 1.
 REPEAT 462 491 ANK 2.
 REPEAT 495 524 ANK 3.
 REPEAT 528 560 ANK 4.
 REPEAT 564 593 ANK 5.
 REPEAT 597 627 ANK 6.
 REPEAT 668 698 ANK 7.
 REPEAT 743 772 ANK 8.
 REPEAT 776 805 ANK 9.
 REPEAT 809 838 ANK 10.
 REPEAT 842 871 ANK 11.
 MOD_RES 1023 1023 Phosphothreonine.  
Keyword
 3D-structure; ANK repeat; Complete proteome; Cytoplasm; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1050 AA 
Protein Sequence
MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST CQFESYILIP 60
VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF YNEKEESFSI LCIAHPLEKR 120
ESSEEPLAPS DPFSLKTIED VREFLGRHSE RFDRNIASFH RTFRECERKS LRHHIDSANA 180
LYTKCLQQLL RDSHLKMLAK QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN 240
KITRSLQDLQ QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ 300
RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSLAKDELGY CLTSFEAAIE 360
YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC LFKHIASGNQ KEVERLLSQE 420
DHDKDTVQKM CHPLCFCDDC EKLVSGRLND PSVVTPFSRD DRGHTPLHVA AVCGQASLID 480
LLVSKGAMVN ATDYHGATPL HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG 540
HEDCVKALVY YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET 600
PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS FSSMSASSRQ 660
EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE DTVSAADPEF CHPLCQCPKC 720
APAQKRLAKV PASGLGVNVT SQDGSSPLHV AALHGRADLI PLLLKHGANA GARNADQAVP 780
LHLACQQGHF QVVKCLLDSN AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN 840
NKGNTALHEA VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV 900
ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG KTSREIMARD 960
RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS DWPERPGLTQ TGPGHRRMLR 1020
RHTVEDAVVS QGPEAAGPLS TPQEVSASRS 1050 
Gene Ontology
 GO:0005769; C:early endosome; IDA:MGI.
 GO:0005764; C:lysosome; IDA:LIFEdb.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:MGI.
 GO:0045022; P:early endosome to late endosome transport; IDA:MGI.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR003123; VPS9.
 IPR013995; VPS9_subgr. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF02204; VPS9 
SMART
 SM00248; ANK
 SM00167; VPS9 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS51205; VPS9 
PRINTS
 PR01415; ANKYRIN.