UniProt Accession

 NENF_HUMAN; Q9UMX5; A1KYQ8; Q53FZ6; Q5TM90 

Genbank Protein ID

 AB126219; AF173937; AY762102; AK223135; CH471100; BC008823 

Genbank Nucleotide ID

 BAD72063.1; AAD51419.1; AAX07829.1; BAD96855.1; EAW93389.1; AAH08823.1 

Protein Name

 Neudesin 

Protein Synonyms/Alias

 Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Secreted protein of unknown function; SPUF protein 

Gene Name

 NENF 

Gene Synonyms/Alias

 CIR2; SPUF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
79	VFDVTSGKEFYGRGA	ubiquitination	[1, 2]
136	FTKVYKAKYPIVGYT	acetylation	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme (By similarity). 

Sequence Annotation

 DOMAIN 44 129 Cytochrome b5 heme-binding.
 MOD_RES 136 136 N6-acetyllysine.  

Keyword

 Acetylation; Complete proteome; Heme; Iron; Metal-binding; Reference proteome; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0043410; P:positive regulation of MAPK cascade; IEA:Compara. 

Interpro

 IPR001199; Cyt_B5-like_heme/steroid-bd. 

Pfam

 PF00173; Cyt-b5 

SMART

  

PROSITE

 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 

PRINTS