UniProt Accession

 MTF2_HUMAN; Q9Y483; A6NGQ9; A8K2Q3; B1AKT5; Q9UES9; Q9UP40 

Genbank Protein ID

 AF072814; AF073293; AJ010014; AK290318; AL117354; AC093577 

Genbank Nucleotide ID

 AAC27618.1; AAC27080.1; CAA08970.1; BAF83007.1; CAI23502.1; CAI23502.1 

Protein Name

 Metal-response element-binding transcription factor 2 

Protein Synonyms/Alias

 Metal regulatory transcription factor 2; Metal-response element DNA-binding protein M96; Polycomb-like protein 2; hPCl2 

Gene Name

 MTF2 

Gene Synonyms/Alias

 PCL2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
161	CVFATTTKRGGALKK	ubiquitination	[1]
313	GELADTPKSERYEHV	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity). 

Sequence Annotation

 DOMAIN 44 101 Tudor.
 ZN_FING 102 157 PHD-type 1.
 ZN_FING 201 255 PHD-type 2.
 MOD_RES 24 24 Phosphothreonine.  

Keyword

 Alternative splicing; Chromatin regulator; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 593 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0035064; F:methylated histone residue binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0061086; P:negative regulation of histone H3-K27 methylation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0061087; P:positive regulation of histone H3-K27 methylation; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0007379; P:segment specification; ISS:UniProtKB.
 GO:0019827; P:stem cell maintenance; ISS:UniProtKB. 

Interpro

 IPR025894; Mtf2_C_dom.
 IPR002999; Tudor.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF14061; Mtf2_C
 PF00628; PHD 

SMART

 SM00249; PHD
 SM00333; TUDOR 

PROSITE

 PS50304; TUDOR
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 

PRINTS