CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024790
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ras/Rap GTPase-activating protein SynGAP 
Protein Synonyms/Alias
 Neuronal RasGAP; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1 
Gene Name
 Syngap1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
339DKKRKKDKAGYVGLVubiquitination[1]
492RENTLATKAIEEYMRubiquitination[1]
507LIGQKYLKDAIGEFIubiquitination[1]
638QNLANFSKFTSKEDFubiquitination[1]
710LSKEALLKLGPLPRLubiquitination[1]
793LPSPTKEKPPPPPPGubiquitination[1]
839QKMLSVNKSVSMLDLubiquitination[1]
1108PRQQSLSKEGSIGGSubiquitination[1]
1193ESRLDRVKEYEEEIHubiquitination[1]
1229SQEEQTSKILMQYQAubiquitination[1]
1257VEKDSQIKSIIGRLMubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR- mediated miniature excitatory postsynaptic currents. Exhibits dual GTPase-activating speciticity for Ras and Rap. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits (By similarity). 
Sequence Annotation
 DOMAIN 150 251 PH.
 DOMAIN 249 347 C2.
 DOMAIN 443 635 Ras-GAP.
 MOTIF 785 815 SH3-binding (Potential).
 MOD_RES 379 379 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 385 385 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 449 449 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 466 466 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 836 836 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 840 840 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 842 842 Phosphoserine; by PLK2 (By similarity).
 MOD_RES 895 895 Phosphoserine; by PLK2 (By similarity).  
Keyword
 Cell junction; Complete proteome; GTPase activation; Membrane; Phosphoprotein; Reference proteome; SH3-binding; Synapse. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1340 AA 
Protein Sequence
MSRSRASIHR GSIPAMSYAP FRDVRGPPMH RTQYVHSPYD RPGWNPRFCI ISGNQLLMLD 60
EDEIHPLLIR DRRSESSRNK LLRRTVSVPV EGRPHGEHEY HLGRSRRKSV PGGKQYSMEA 120
APAAPFRPSQ GFLSRRLKSS IKRTKSQPKL DRTSSFRQIL PRFRSADHDR ARLMQSFKES 180
HSHESLLSPS SAAEALELNL DEDSIIKPVH SSILGQEFCF EVTTSSGTKC FACRSAAERD 240
KWIENLQRAV KPNKDNSRRV DNVLKLWIIE ARELPPKKRY YCELCLDDML YARTTSKPRS 300
ASGDTVFWGE HFEFNNLPAV RALRLHLYRD SDKKRKKDKA GYVGLVTVPV ATLAGRHFTE 360
QWYPVTLPTG SGGSGGMGSG GGGGSGGGSG GKGKGGCPAV RLKARYQTMS ILPMELYKEF 420
AEYVTNHYRM LCAVLEPALN VKGKEEVASA LVHILQSTGK AKDFLSDMAM SEVDRFMERE 480
HLIFRENTLA TKAIEEYMRL IGQKYLKDAI GEFIRALYES EENCEVDPIK CTASSLAEHQ 540
ANLRMCCELA LCKVVNSHCV FPRELKEVFA SWRLRCAERG REDIADRLIS ASLFLRFLCP 600
AIMSPSLFGL MQEYPDEQTS RTLTLIAKVI QNLANFSKFT SKEDFLGFMN EFLELEWGSM 660
QQFLYEISNL DTLTNSSSFE GYIDLGRELS TLHALLWEVL PQLSKEALLK LGPLPRLLND 720
ISTALRNPNI QRQPSRQSER TRSQPMVLRG PSAEMQGYMM RDLNSSIDLQ SFMARGLNSS 780
MDMARLPSPT KEKPPPPPPG GGKDLFYVSR PPLARSSPAY CTSSSDITEP EQKMLSVNKS 840
VSMLDLQGDG PGGRLNSSSV SNLAAVGDLL HSSQASLTAA LGLRPAPAGR LSQGSGSSIT 900
AAGMRLSQMG VTTDGVPAQQ LRIPLSFQNP LFHMAADGPG PPAGHGGSSG HGPPSSHHHH 960
HHHHHHRGGE PPGDTFAPFH GYSKSEDLSS GVPKPPAASI LHSHSYSDEF GPSGTDFTRR 1020
QLSLQDSLQH MLSPPQITIG PQRPAPSGPG GGSGGGSGGG QPPPLQRGKS QQLTVSAAQK 1080
PRPSSGNLLQ SPEPSYGPAR PRQQSLSKEG SIGGSGGSGG GGGGGLKPSI TKQHSQTPST 1140
LNPTMPASER TVAWVSNMPH LSADIESAHI EREEYKLKEY SKSMDESRLD RVKEYEEEIH 1200
SLKERLHMSN RKLEEYERRL LSQEEQTSKI LMQYQARLEQ SEKRLRQQQV EKDSQIKSII 1260
GRLMLVEEEL RRDHPAMAEP LPEPKKRLLD AQERQLPPLG PTNPRVTLAP PWNGLAPPAP 1320
PPPPRLQITE NGEFRNTADH 1340 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0043198; C:dendritic shaft; IDA:MGI.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IBA:RefGenome.
 GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005097; F:Rab GTPase activator activity; IDA:MGI.
 GO:0016358; P:dendrite development; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0050771; P:negative regulation of axonogenesis; IDA:MGI.
 GO:0046580; P:negative regulation of Ras protein signal transduction; ISS:UniProtKB.
 GO:0007389; P:pattern specification process; IMP:MGI.
 GO:0007265; P:Ras protein signal transduction; IDA:MGI.
 GO:0043113; P:receptor clustering; IMP:MGI.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IMP:MGI.
 GO:0043408; P:regulation of MAPK cascade; IMP:MGI.
 GO:0050803; P:regulation of synapse structure and activity; IMP:MGI.
 GO:0008542; P:visual learning; IMP:MGI. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR021887; DUF3498.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR023315; SynGAP_C2_N. 
Pfam
 PF00168; C2
 PF12004; DUF3498
 PF00616; RasGAP 
SMART
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2 
PRINTS