CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008930
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 12 member 2 
Protein Synonyms/Alias
 Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 
Gene Name
 SLC12A2 
Gene Synonyms/Alias
 NKCC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
114AGAGAGAKQTPADGEubiquitination[1]
130SGESEPAKGSEEAKGubiquitination[1]
136AKGSEEAKGRFRVNFubiquitination[2]
237TAAQLGEKLLRPSLAubiquitination[2, 3, 4]
252ELHDELEKEPFEDGFubiquitination[1, 2]
280VTYTAESKGVVKFGWubiquitination[1, 2]
785SGVEDHVKNFRPQCLubiquitination[1]
833GPRRQAMKEMSIDQAubiquitination[2, 5]
841EMSIDQAKYQRWLIKubiquitination[5]
848KYQRWLIKNKMKAFYubiquitination[5]
850QRWLIKNKMKAFYAPubiquitination[5]
923GVVVIRLKEGLDISHubiquitination[1, 2]
943ELLSSQEKSPGTKDVubiquitination[1, 2, 6]
948QEKSPGTKDVVVSVEubiquitination[1, 2, 6, 7]
958VVSVEYSKKSDLDTSubiquitination[1, 2]
959VSVEYSKKSDLDTSKubiquitination[1, 2]
966KSDLDTSKPLSEKPIubiquitination[1, 2]
971TSKPLSEKPITHKVEubiquitination[1, 2]
976SEKPITHKVEEEDGKubiquitination[1, 2]
983KVEEEDGKTATQPLLubiquitination[1, 2]
991TATQPLLKKESKGPIubiquitination[1, 2]
995PLLKKESKGPIVPLNubiquitination[1, 2, 5]
1007PLNVADQKLLEASTQubiquitination[1, 2, 3, 4]
1017EASTQFQKKQGKNTIubiquitination[1, 2]
1061IRVFIGGKINRIDHDubiquitination[1, 2, 5]
1078AMATLLSKFRIDFSDubiquitination[5]
1095VLGDINTKPKKENIIubiquitination[1, 2]
1098DINTKPKKENIIAFEubiquitination[1, 2, 6]
1118YRLHEDDKEQDIADKubiquitination[1, 2]
1125KEQDIADKMKEDEPWubiquitination[1]
1143DNELELYKTKTYRQIubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. 
Sequence Annotation
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 79 79 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Ion transport; Membrane; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1212 AA 
Protein Sequence
MEPRPTAPSS GAPGLAGVGE TPSAAALAAA RVELPGTAVP SVPEDAAPAS RDGGGVRDEG 60
PAAAGDGLGR PLGPTPSQSR FQVDLVSENA GRAAAAAAAA AAAAAAAGAG AGAKQTPADG 120
EASGESEPAK GSEEAKGRFR VNFVDPAASS SAEDSLSDAA GVGVDGPNVS FQNGGDTVLS 180
EGSSLHSGGG GGSGHHQHYY YDTHTNTYYL RTFGHNTMDA VPRIDHYRHT AAQLGEKLLR 240
PSLAELHDEL EKEPFEDGFA NGEESTPTRD AVVTYTAESK GVVKFGWIKG VLVRCMLNIW 300
GVMLFIRLSW IVGQAGIGLS VLVIMMATVV TTITGLSTSA IATNGFVRGG GAYYLISRSL 360
GPEFGGAIGL IFAFANAVAV AMYVVGFAET VVELLKEHSI LMIDEINDIR IIGAITVVIL 420
LGISVAGMEW EAKAQIVLLV ILLLAIGDFV IGTFIPLESK KPKGFFGYKS EIFNENFGPD 480
FREEETFFSV FAIFFPAATG ILAGANISGD LADPQSAIPK GTLLAILITT LVYVGIAVSV 540
GSCVVRDATG NVNDTIVTEL TNCTSAACKL NFDFSSCESS PCSYGLMNNF QVMSMVSGFT 600
PLISAGIFSA TLSSALASLV SAPKIFQALC KDNIYPAFQM FAKGYGKNNE PLRGYILTFL 660
IALGFILIAE LNVIAPIISN FFLASYALIN FSVFHASLAK SPGWRPAFKY YNMWISLLGA 720
ILCCIVMFVI NWWAALLTYV IVLGLYIYVT YKKPDVNWGS STQALTYLNA LQHSIRLSGV 780
EDHVKNFRPQ CLVMTGAPNS RPALLHLVHD FTKNVGLMIC GHVHMGPRRQ AMKEMSIDQA 840
KYQRWLIKNK MKAFYAPVHA DDLREGAQYL MQAAGLGRMK PNTLVLGFKK DWLQADMRDV 900
DMYINLFHDA FDIQYGVVVI RLKEGLDISH LQGQEELLSS QEKSPGTKDV VVSVEYSKKS 960
DLDTSKPLSE KPITHKVEEE DGKTATQPLL KKESKGPIVP LNVADQKLLE ASTQFQKKQG 1020
KNTIDVWWLF DDGGLTLLIP YLLTTKKKWK DCKIRVFIGG KINRIDHDRR AMATLLSKFR 1080
IDFSDIMVLG DINTKPKKEN IIAFEEIIEP YRLHEDDKEQ DIADKMKEDE PWRITDNELE 1140
LYKTKTYRQI RLNELLKEHS STANIIVMSL PVARKGAVSS ALYMAWLEAL SKDLPPILLV 1200
RGNHQSVLTF YS 1212 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0051739; F:ammonia transmembrane transporter activity; IDA:UniProtKB.
 GO:0008511; F:sodium:potassium:chloride symporter activity; TAS:ProtInc.
 GO:0060444; P:branching involved in mammary gland duct morphogenesis; IEA:Compara.
 GO:0050910; P:detection of mechanical stimulus involved in sensory perception of sound; IEA:Compara.
 GO:0060763; P:mammary duct terminal end bud growth; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
 GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
 GO:0070634; P:transepithelial ammonium transport; IDA:UniProtKB.
 GO:0030321; P:transepithelial chloride transport; IDA:UniProtKB. 
Interpro
 IPR004841; AA-permease_dom.
 IPR013612; AA_permease_N.
 IPR002443; Na/K/Cl_cotranspt.
 IPR002444; Na/K/Cl_cotranspt1.
 IPR004842; Na/K/Cl_cotransptS. 
Pfam
 PF00324; AA_permease
 PF08403; AA_permease_N 
SMART
  
PROSITE
  
PRINTS
 PR01207; NAKCLTRNSPRT.
 PR01208; NAKCLTRSPRT1.