UniProt Accession

 INMT_MOUSE; P40936; Q9CZ50 

Genbank Protein ID

 M88694; AK002281; AK013010; BC013518 

Genbank Nucleotide ID

 AAA62365.1; BAB21985.1; BAB28594.1; AAH13518.1 

Protein Name

 Indolethylamine N-methyltransferase 

Protein Synonyms/Alias

 Indolamine N-methyltransferase; Aromatic alkylamine N-methyltransferase; Amine N-methyltransferase; Arylamine N-methyltransferase; Thioether S-methyltransferase; TEMT 

Gene Name

 Inmt 

Gene Synonyms/Alias

 Temt 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
14	IGGEDYEKEFTPKDY	acetylation	[1]
14	IGGEDYEKEFTPKDY	succinylation	[1]
14	IGGEDYEKEFTPKDY	ubiquitination	[2]
19	YEKEFTPKDYLTTYY	ubiquitination	[2]
97	QNLQELQKWLKKEPG	acetylation	[1, 3]
97	QNLQELQKWLKKEPG	succinylation	[1]
97	QNLQELQKWLKKEPG	ubiquitination	[2]
101	ELQKWLKKEPGAYDW	ubiquitination	[2]
211	HYMVGPKKFSGVYLE	acetylation	[1, 4]
211	HYMVGPKKFSGVYLE	succinylation	[1]
211	HYMVGPKKFSGVYLE	ubiquitination	[2]
219	FSGVYLEKEVVEKAI	acetylation	[3, 4, 5, 6]
219	FSGVYLEKEVVEKAI	ubiquitination	[2]
224	LEKEVVEKAIQDAGC	acetylation	[3, 4, 6]
224	LEKEVVEKAIQDAGC	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Catalyzes the N-methylation of tryptamine and structurally related compounds (By similarity). Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n- propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds. 

Sequence Annotation

 REGION 64 65 S-adenosyl-L-methionine binding (By
 REGION 143 144 S-adenosyl-L-methionine binding (By
 BINDING 21 21 S-adenosyl-L-methionine (By similarity).
 BINDING 26 26 S-adenosyl-L-methionine (By similarity).
 BINDING 70 70 S-adenosyl-L-methionine (By similarity).
 BINDING 86 86 S-adenosyl-L-methionine (By similarity).
 BINDING 91 91 S-adenosyl-L-methionine (By similarity).
 BINDING 164 164 S-adenosyl-L-methionine; via carbonyl  

Keyword

 Complete proteome; Cytoplasm; Detoxification; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0030748; F:amine N-methyltransferase activity; ISS:UniProtKB.
 GO:0004790; F:thioether S-methyltransferase activity; IDA:MGI.
 GO:0009308; P:amine metabolic process; ISS:UniProtKB.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW. 

Interpro

 IPR025817; Amine_MeTrfase.
 IPR025820; NNMT/PNMT/TEMT_CS.
 IPR000940; NNMT_TEMT_trans. 

Pfam

 PF01234; NNMT_PNMT_TEMT 

SMART

  

PROSITE

 PS01100; NNMT_PNMT_TEMT 

PRINTS