CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002041
UniProt Accession
Genbank Protein ID
 X02346 
Genbank Nucleotide ID
Protein Name
 Replication protein E1 
Protein Synonyms/Alias
 ATP-dependent helicase E1 
Gene Name
 E1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Bovine papillomavirus type 1 
NCBI Taxa ID
 10559 
Lysine Modification
Position
Peptide
Type
References
514HKAAVQIKAPPLLVTsumoylation[1, 2, 3]
Reference
 [1] SUMO-1 modification of bovine papillomavirus E1 protein is required for intranuclear accumulation.
 Rangasamy D, Woytek K, Khan SA, Wilson VG.
 J Biol Chem. 2000 Dec 1;275(48):37999-8004. [PMID: 11005821]
 [2] Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin.
 Rallabhandi P, Hashimoto K, Mo YY, Beck WT, Moitra PK, D'Arpa P.
 J Biol Chem. 2002 Oct 18;277(42):40020-6. [PMID: 12149243]
 [3] Characterization of papillomavirus E1 helicase mutants defective for interaction with the SUMO-conjugating enzyme Ubc9.
 Fradet-Turcotte A, Brault K, Titolo S, Howley PM, Archambault J.
 Virology. 2009 Dec 20;395(2):190-201. [PMID: 19836047] 
Functional Description
 ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. 
Sequence Annotation
 DOMAIN 407 557 SF3 helicase.
 NP_BIND 433 440 ATP (Potential).  
Keyword
 3D-structure; ATP-binding; Complete proteome; DNA replication; DNA-binding; Early protein; Helicase; Host nucleus; Hydrolase; Nucleotide-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 605 AA 
Protein Sequence
MANDKGSNWD SGLGCSYLLT EAECESDKEN EEPGAGVELS VESDRYDSQD EDFVDNASVF 60
QGNHLEVFQA LEKKAGEEQI LNLKRKVLGS SQNSSGSEAS ETPVKRRKSG AKRRLFAENE 120
ANRVLTPLQV QGEGEGRQEL NEEQAISHLH LQLVKSKNAT VFKLGLFKSL FLCSFHDITR 180
LFKNDKTTNQ QWVLAVFGLA EVFFEASFEL LKKQCSFLQM QKRSHEGGTC AVYLICFNTA 240
KSRETVRNLM ANTLNVREEC LMLQPAKIRG LSAALFWFKS SLSPATLKHG ALPEWIRAQT 300
TLNESLQTEK FDFGTMVQWA YDHKYAEESK IAYEYALAAG SDSNARAFLA TNSQAKHVKD 360
CATMVRHYLR AETQALSMPA YIKARCKLAT GEGSWKSILT FFNYQNIELI TFINALKLWL 420
KGIPKKNCLA FIGPPNTGKS MLCNSLIHFL GGSVLSFANH KSHFWLASLA DTRAALVDDA 480
THACWRYFDT YLRNALDGYP VSIDRKHKAA VQIKAPPLLV TSNIDVQAED RYLYLHSRVQ 540
TFRFEQPCTD ESGEQPFNIT DADWKSFFVR LWGRLDLIDE EEDSEEDGDS MRTFTCSARN 600
TNAVD 605 
Gene Ontology
 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW. 
Interpro
 IPR001177; DNA_helicase_E1_C_Papillomavir.
 IPR014000; DNA_helicase_E1_N_Papillomavir.
 IPR014015; Helicase_SF3_DNA-vir.
 IPR027417; P-loop_NTPase.
 IPR016393; Rep_E1_papillomaV. 
Pfam
 PF00519; PPV_E1_C
 PF00524; PPV_E1_N 
SMART
  
PROSITE
 PS51206; SF3_HELICASE_1 
PRINTS