CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005770
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SHC-transforming protein 1 
Protein Synonyms/Alias
 SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1 
Gene Name
 SHC1 
Gene Synonyms/Alias
 SHC; SHCA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
203CEAVPGAKGATRRRKubiquitination[1, 2]
313QAFELRFKQYLRNPPubiquitination[1]
462PRDLFDMKPFEDALRubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. 
Sequence Annotation
 DOMAIN 156 339 PID.
 DOMAIN 488 579 SH2.
 REGION 340 487 CH1.
 MOD_RES 36 36 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 349 349 Phosphotyrosine.
 MOD_RES 350 350 Phosphotyrosine.
 MOD_RES 426 426 Phosphoserine.
 MOD_RES 427 427 Phosphotyrosine.  
Keyword
 3D-structure; Alternative promoter usage; Alternative splicing; Angiogenesis; Complete proteome; Cytoplasm; Growth regulation; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 583 AA 
Protein Sequence
MDLLPPKPKY NPLRNESLSS LEEGASGSTP PEELPSPSAS SLGPILPPLP GDDSPTTLCS 60
FFPRMSNLRL ANPAGGRPGS KGEPGRAADD GEGIVGAAMP DSGPLPLLQD MNKLSGGGGR 120
RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD 180
FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS 240
LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV 300
ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL 360
GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPGRE 420
LFDDPSYVNV QNLDKARQAV GGAGPPNPAI NGSAPRDLFD MKPFEDALRV PPPPQSVSMA 480
EQLRGEPWFH GKLSRREAEA LLQLNGDFLV RESTTTPGQY VLTGLQSGQP KHLLLVDPEG 540
VVRTKDHRFE SVSHLISYHM DNHLPIISAG SELCLQQPVE RKL 583 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0070435; C:Shc-EGFR complex; ISS:BHF-UCL.
 GO:0005154; F:epidermal growth factor receptor binding; ISS:BHF-UCL.
 GO:0005543; F:phospholipid binding; TAS:UniProtKB.
 GO:0004713; F:protein tyrosine kinase activity; TAS:Reactome.
 GO:0005068; F:transmembrane receptor protein tyrosine kinase adaptor activity; TAS:UniProtKB.
 GO:0031532; P:actin cytoskeleton reorganization; IEA:Compara.
 GO:0000187; P:activation of MAPK activity; IDA:UniProtKB.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0016337; P:cell-cell adhesion; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; ISS:BHF-UCL.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0000165; P:MAPK cascade; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; NAS:UniProtKB.
 GO:0045740; P:positive regulation of DNA replication; ISS:BHF-UCL.
 GO:0007265; P:Ras protein signal transduction; TAS:Reactome.
 GO:0007176; P:regulation of epidermal growth factor-activated receptor activity; TAS:ProtInc.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW. 
Interpro
 IPR011993; PH_like_dom.
 IPR006019; PID_domain.
 IPR006020; PTyr_interaction_dom.
 IPR000980; SH2. 
Pfam
 PF00640; PID
 PF00017; SH2 
SMART
 SM00462; PTB
 SM00252; SH2 
PROSITE
 PS01179; PID
 PS50001; SH2 
PRINTS
 PR00401; SH2DOMAIN.
 PR00629; SHCPIDOMAIN.