CPLM-008356

Genbank Nucleotide ID

Ras-related protein Rab-5C

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
117	ARAKNWVKELQRQAS	ubiquitination	[1, 2, 3, 4, 5]
135	VIALAGNKADLASKR	ubiquitination	[2, 3, 6]
141	NKADLASKRAVEFQE	ubiquitination	[3, 5, 6]
180	EIFMAIAKKLPKNEP	ubiquitination	[3, 6, 7]
184	AIAKKLPKNEPQNAT	acetylation	[8]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Protein transport. Probably involved in vesicular traffic (By similarity).

NP_BIND 28 35 GTP (By similarity).
NP_BIND 76 80 GTP (By similarity).
NP_BIND 134 137 GTP (By similarity).
MOTIF 50 58 Effector region (Potential).
LIPID 213 213 S-geranylgeranyl cysteine (By
LIPID 214 214 S-geranylgeranyl cysteine (By

Alternative splicing; Cell membrane; Complete proteome; Endosome; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Polymorphism; Prenylation; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
GO:0005811; C:lipid particle; IDA:UniProtKB.
GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0019003; F:GDP binding; IDA:UniProtKB.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; TAS:ProtInc.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003579; Small_GTPase_Rab_type.

PR00449; RASTRNSFRMNG.