UniProt Accession

 GSTM4_HUMAN; Q03013; A8K765; Q05465; Q32NC1; Q6FH87 

Genbank Protein ID

 M96234; M96233; X68677; M99422; CR541869; AK291880; CH471122; BC015513; BC108729; X56837 

Genbank Nucleotide ID

 AAA57347.1; AAA57346.1; CAA48637.1; AAA58623.1; CAG46667.1; BAF84569.1; EAW56405.1; AAH15513.1; AAI08730.1; CAA40167.1 

Protein Name

 Glutathione S-transferase Mu 4 

Protein Synonyms/Alias

 GST class-mu 4; GST-Mu2; GSTM4-4 

Gene Name

 GSTM4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
31	TDSSYEEKKYTMGDA	ubiquitination	[1]
50	RSQWLNEKFKLGLDF	ubiquitination	[1]
52	QWLNEKFKLGLDFPN	ubiquitination	[1]
182	LDAFPNLKDFISRFE	ubiquitination	[1, 2, 3]
193	SRFEGLEKISAYMKS	ubiquitination	[1]
199	EKISAYMKSSRFLPK	ubiquitination	[1]
206	KSSRFLPKPLYTRVA	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1- chloro-2,4-dinitrobenzene. 

Sequence Annotation

 DOMAIN 2 88 GST N-terminal.
 DOMAIN 90 208 GST C-terminal.
 REGION 7 8 Glutathione binding (By similarity).
 REGION 46 50 Glutathione binding (By similarity).
 REGION 59 60 Glutathione binding (By similarity).
 REGION 72 73 Glutathione binding (By similarity).
 BINDING 116 116 Substrate (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 218 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0043295; F:glutathione binding; IDA:BHF-UCL.
 GO:0004364; F:glutathione transferase activity; IDA:BHF-UCL.
 GO:0006749; P:glutathione metabolic process; IDA:BHF-UCL.
 GO:0042178; P:xenobiotic catabolic process; IDA:BHF-UCL. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C.
 IPR003081; GST_mu.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C
 PF02798; GST_N 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS

 PR01267; GSTRNSFRASEM.