CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014235
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein SATB1 
Protein Synonyms/Alias
 Special AT-rich sequence-binding protein 1 
Gene Name
 Satb1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
65KHSGHLMKTNLRKGTubiquitination[1]
232YANVSAAKCQEFGRWubiquitination[1]
Reference
 [1] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144] 
Functional Description
 Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold- associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin- loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. 
Sequence Annotation
 DNA_BIND 361 448 CUT 1.
 DNA_BIND 484 571 CUT 2.
 DNA_BIND 646 705 Homeobox.
 REGION 90 204 PDZ-like dimerization domain.
 REGION 224 278 Nuclear matrix targeting sequence (NMTS)
 REGION 400 410 Matrix attachment region (MAR) DNA-
 MOTIF 20 40 Nuclear localization signal (By
 MOTIF 139 143 Protein interaction (By similarity).
 MOTIF 224 278 Nuclear matrix targeting sequence (NMTS).
 BINDING 390 390 Matrix attachment region (MAR) DNA (By
 BINDING 425 425 Matrix attachment region (MAR) DNA (By
 MOD_RES 136 136 N6-acetyllysine (By similarity).
 MOD_RES 185 185 Phosphoserine (By similarity).
 CROSSLNK 745 745 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Chromatin regulator; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; DNA-binding; Homeobox; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 764 AA 
Protein Sequence
MDHLNEATQG KEHSEMSNNV SDPKGPPAKI ARLEQNGSPL GRGRLGSTGG KMQGVPLKHS 60
GHLMKTNLRK GTMLPVFCVV EHYENAIEYD CKEEHAEFVL VRKDMLFNQL IEMALLSLGY 120
SHSSAAQAKG LIQVGKWNPV PLSYVTDAPD ATVADMLQDV YHVVTLKIQL HSCPKLEDLP 180
PEQWSHTTVR NALKDLLKDM NQSSLAKECP LSQSMISSIV NSTYYANVSA AKCQEFGRWY 240
KHFKKTKDMM VEMDSLSELS QQGANHVNFG QQPVPGNTAE QPPSPAQLSH GSQPSVRTPL 300
PNLHPGLVST PISPQLVNQQ LVMAQLLNQQ YAVNRLLAQQ SLNQQYLNHP PPVSRSMNKP 360
LEQQVSTNTE VSSEIYQWVR DELKRAGISQ AVFARVAFNR TQGLLSEILR KEEDPKTASQ 420
SLLVNLRAMQ NFLQLPEAER DRIYQDERER SLNAASAMGP APLLSTPPSR PPQVKTATLA 480
TERNGKPENN TMNINASIYD EIQQEMKRAK VSQALFAKVA ATKSQGWLCE LLRWKEDPSP 540
ENRTLWENLS MIRRFLSLPQ PERDAIYEQE SNAVHHHGDR PPHIIHVPAE QIQQQQQQQQ 600
QQQQQQQPPP PPPQPQPQPQ AGPRLPPRQP TVASSAESDE ENRQKTRPRT KISVEALGIL 660
QSFIQDVGLY PDEEAIQTLS AQLDLPKYTI IKFFQNQRYY LKHHGKLKDN SGLEVDVAEY 720
KDEELLKDLE ESVQDKNANT LFSVKLEEEL SVEGSTDVNA DLKD 764 
Gene Ontology
 GO:0005720; C:nuclear heterochromatin; IDA:MGI.
 GO:0016363; C:nuclear matrix; IDA:MGI.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0050798; P:activated T cell proliferation; IMP:MGI.
 GO:0043367; P:CD4-positive, alpha-beta T cell differentiation; IMP:MGI.
 GO:0043374; P:CD8-positive, alpha-beta T cell differentiation; IMP:MGI.
 GO:0006338; P:chromatin remodeling; IMP:MGI.
 GO:0008544; P:epidermis development; IMP:MGI.
 GO:0016571; P:histone methylation; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0060004; P:reflex; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003350; Hmoeo_CUT.
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR010982; Lambda_DNA-bd_dom. 
Pfam
 PF02376; CUT
 PF00046; Homeobox 
SMART
 SM00389; HOX 
PROSITE
 PS51042; CUT
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2 
PRINTS