CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018169
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vigilin 
Protein Synonyms/Alias
 High density lipoprotein-binding protein; HDL-binding protein 
Gene Name
 Hdlbp 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
41ENDPPTYKDAFPPLPubiquitination[1]
67PAGAWSNKIRPIKASacetylation[2]
90PLEERKYKDMNQFGEubiquitination[1]
168HHRFVIGKNGEKLQDubiquitination[1]
221LISAEQDKRAVERLEubiquitination[1]
231VERLEVEKAFHPFIAubiquitination[1]
274EIVFTGEKEQLAQAVubiquitination[1]
303TTIAVEVKKSQHKYVubiquitination[1]
314HKYVIGPKGNSLQEIubiquitination[1]
349ILRGEPEKLGQALTEubiquitination[1]
389KKGQNLAKITQQMPKubiquitination[1]
453FHRHLIGKSGANINRacetylation[2, 3]
494PQGVQQAKRELLELAacetylation[4]
494PQGVQQAKRELLELAubiquitination[1]
536RIREIRDKFPEVIINacetylation[5]
599FHKNIIGKGGANIKKubiquitination[1]
663VEVSIPAKLHNSLIGubiquitination[1]
672HNSLIGTKGRLIRSIubiquitination[1]
714SDVEKAKKQLLHLAEubiquitination[1]
723LLHLAEEKQTKSFTVacetylation[3]
745YHKFLIGKGGGKIRKubiquitination[1]
779DLITIIGKEDAVREAubiquitination[1]
853KVTLKGAKDCVEAAKubiquitination[1]
991HRYIIGQKGSGIRKMacetylation[3]
991HRYIIGQKGSGIRKMubiquitination[1]
1039AGLLDRVKELQAEQEubiquitination[1]
1062LSVTVDPKYHPKIIGubiquitination[1]
1066VDPKYHPKIIGRKGAubiquitination[1]
1108ITITGYEKNTEAARDubiquitination[1]
1224EESRAPSKGFVVRDAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol (By similarity). 
Sequence Annotation
 DOMAIN 150 212 KH 1.
 DOMAIN 222 284 KH 2.
 DOMAIN 295 357 KH 3.
 DOMAIN 364 424 KH 4.
 DOMAIN 435 497 KH 5.
 DOMAIN 507 570 KH 6.
 DOMAIN 581 643 KH 7.
 DOMAIN 653 716 KH 8.
 DOMAIN 727 790 KH 9.
 DOMAIN 800 863 KH 10.
 DOMAIN 873 967 KH 11.
 DOMAIN 972 1034 KH 12.
 DOMAIN 1052 1117 KH 13.
 DOMAIN 1127 1190 KH 14.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 295 295 Phosphothreonine (Potential).
 MOD_RES 296 296 Phosphothreonine (Potential).
 MOD_RES 437 437 Phosphotyrosine (By similarity).  
Keyword
 Acetylation; Cholesterol metabolism; Complete proteome; Cytoplasm; HDL; Lipid metabolism; Lipid transport; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Steroid metabolism; Sterol metabolism; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1268 AA 
Protein Sequence
MSSVAVLTQE SFAEHRSGLV PQQIKVATLN SEEENDPPTY KDAFPPLPEK AACLESAQEP 60
AGAWSNKIRP IKASVITQVF HVPLEERKYK DMNQFGEGEQ AKICLEIMQR TGAHLELSLA 120
KDQGLSIMVS GKLDAVMKAR KDIVARLQTQ ASATVPIPKE HHRFVIGKNG EKLQDLELKT 180
ATKIQIPRPD DPSNQIKITG TKEGIEKARH EVLLISAEQD KRAVERLEVE KAFHPFIAGP 240
YNRLVGEIMQ ETGTRINIPP PSVNRTEIVF TGEKEQLAQA VARIKKIYEE KKKKTTTIAV 300
EVKKSQHKYV IGPKGNSLQE ILERTGVSVE IPPSDSISET VILRGEPEKL GQALTEVYAK 360
ANSFTVSSVS APSWLHRFII GKKGQNLAKI TQQMPKVHIE FTEGEDKITL EGPTEDVNVA 420
QEQIEGMVKD LINRMDYVEI NIDHKFHRHL IGKSGANINR IKDQYKVSVR IPPDSEKSNL 480
IRIEGDPQGV QQAKRELLEL ASRMENERTK DLIIEQRFHR TIIGQKGERI REIRDKFPEV 540
IINFPDPAQK SDIVQLRGPK NEVEKCTKYM QKMVADLVEN SYSISVPIFK QFHKNIIGKG 600
GANIKKIREE SNTKIDLPAE NSNSETIIIT GKRANCEAAR SRILSIQKDL ANIAEVEVSI 660
PAKLHNSLIG TKGRLIRSIM EECGGVHIHF PVEGSGSDTV VIRGPSSDVE KAKKQLLHLA 720
EEKQTKSFTV DIRAKPEYHK FLIGKGGGKI RKVRDSTGAR IIFPAAEDKD QDLITIIGKE 780
DAVREAQKEL EALIQNLENV VEDYMLVDPK HHRHFVIRRG QVLREIAEEY GGVMVSFPRS 840
GTQSDKVTLK GAKDCVEAAK KRIQEIIEDL EAQVTVECAI PQKFHRSVMG PKGSRIQQIT 900
RDYNVQIKFP DREENPVHSV EPSIQENGDE AGEGREAKET DPGSPRRCDI IIISGRKEKC 960
EAAKEALEAL VPVTIEVEVP FDLHRYIIGQ KGSGIRKMMD EFEVNIHVPA PELQSDTIAI 1020
TGLAANLDRA KAGLLDRVKE LQAEQEDRAL RSFKLSVTVD PKYHPKIIGR KGAVITQIRL 1080
EHEVNIQFPD KDDGNQPQDQ ITITGYEKNT EAARDAILKI VGELEQMVSE DVPLDHRVHA 1140
RIIGARGKAI RKIMDEFKVD IRFPQSGAPD PNCVTVTGLP ENVEEAIDHI LNLEEEYLAD 1200
VVDSEALQVY MKPPAHEESR APSKGFVVRD APWTSNSSEK APDMSSSEEF PSFGAQVAPK 1260
TLPWGPKR 1268 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW. 
Interpro
 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1. 
Pfam
 PF00013; KH_1 
SMART
 SM00322; KH 
PROSITE
 PS50084; KH_TYPE_1 
PRINTS