CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007793
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Large proline-rich protein BAG6 
Protein Synonyms/Alias
 BAG family molecular chaperone regulator 6; BCL2-associated athanogene 6; BAG-6; BAG6; HLA-B-associated transcript 3; Protein G3; Protein Scythe 
Gene Name
 BAG6 
Gene Synonyms/Alias
 BAT3; G3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40VGAQMNVKEFKEHIAubiquitination[1]
56SVSIPSEKQRLIYQGubiquitination[1]
80QEYNVGGKVIHLVERubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. 
Sequence Annotation
 DOMAIN 17 92 Ubiquitin-like.
 REPEAT 242 270 1.
 REPEAT 415 443 2.
 REPEAT 574 602 3.
 REPEAT 608 636 4.
 REGION 242 636 4 X 29 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 350 350 Phosphothreonine.
 MOD_RES 964 964 Phosphoserine.
 MOD_RES 973 973 Phosphoserine.
 MOD_RES 1081 1081 Phosphoserine.
 MOD_RES 1117 1117 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; Chaperone; Chromatin regulator; Complete proteome; Cytoplasm; Differentiation; Immunity; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Spermatogenesis; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1132 AA 
Protein Sequence
MEPNDSTSTA VEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 60
YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTHLPSGASS GTGSASATHG GGSPPGTRGP 120
GASVHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 180
SRMECRGGPQ PQHSQPPPQP PAVTPEPVAL SSQTSEPVES EAPPREPMEA EEVEERAPAQ 240
NPELTPGPAP AGPTPAPETN APNHPSPAEY VEVLQELQRL ESRLQPFLQR YYEVLGAAAT 300
TDYNNNHEGR EEDQRLINLV GESLRLLGNT FVALSDLRCN LACTPPRHLH VVRPMSHYTT 360
PMVLQQAAIP IQINVGTTVT MTGNGTRPPP TPNAEAPPPG PGQASSVAPS STNVESSAEG 420
APPPGPAPPP ATSHPRVIRI SHQSVEPVVM MHMNIQDSGT QPGGVPSAPT GPLGPPGHGQ 480
TLGQQVPGFP TAPTRVVIAR PTPPQARPSH PGGPPVSGTL QGAGLGTNAS LAQMVSGLVG 540
QLLMQPVLVA QGTPGMAPPP APATASASAG TTNTATTAGP APGGPAQPPP TPQPSMADLQ 600
FSQLLGNLLG PAGPGAGGSG VASPTITVAM PGVPAFLQGM TDFLQATQTA PPPPPPPPPP 660
PPAPEQQTMP PPGSPSGGAG SPGGLGLESL SPEFFTSVVQ GVLSSLLGSL GARAGSSESI 720
AAFIQRLSGS SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR 780
SFFHQHYLGG QEPTPSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR TNLEFLQEQF 840
NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG QQMELAAVIN GRIRRMSRGV 900
NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI LRYVRRVGDP PQPLPEEPME VQGAERASPE 960
PQRENASPAP GTTAEEAMSR GPPPAPEGGS RDEQDGASAE TEPWAAAVPP EWVPIIQQDI 1020
QSQRKVKPQP PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS 1080
RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAQR AFADDP 1126 
Gene Ontology
 GO:0071818; C:BAT3 complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
 GO:0070628; F:proteasome binding; ISS:UniProtKB.
 GO:0043022; F:ribosome binding; IDA:UniProtKB.
 GO:0007420; P:brain development; ISS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:0018393; P:internal peptidyl-lysine acetylation; IDA:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IMP:UniProtKB.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; ISS:UniProtKB.
 GO:0001822; P:kidney development; ISS:UniProtKB.
 GO:0030324; P:lung development; ISS:UniProtKB.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
 GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IDA:UniProtKB.
 GO:0006810; P:transport; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR021925; DUF3538.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12057; DUF3538
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS