UniProt Accession

 H2AZ_YEAST; Q12692; D6W255 

Genbank Protein ID

 Z74754; AY558000; BK006948 

Genbank Nucleotide ID

 CAA99011.1; AAS56326.1; DAA10771.1 

Protein Name

 Histone H2A.Z 

Protein Synonyms/Alias

  

Gene Name

 HTZ1 

Gene Synonyms/Alias

 H2AZ; HTA3; YOL012C; O2345 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
4	****MSGKAHGGKGK	acetylation	[1, 2]
9	SGKAHGGKGKSGAKD	acetylation	[1, 2]
11	KAHGGKGKSGAKDSG	acetylation	[1, 2]
15	GKGKSGAKDSGSLRS	acetylation	[1, 2, 3]
121	GVLPHINKALLLKVE	acetylation	[2]
126	INKALLLKVEKKGSK	acetylation	[2]
126	INKALLLKVEKKGSK	sumoylation	[4]
133	KVEKKGSKK******	sumoylation	[4]

Reference

 [1] Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast.
 Millar CB, Xu F, Zhang K, Grunstein M.
 Genes Dev. 2006 Mar 15;20(6):711-22. [PMID: 16543223]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4.
 Keogh MC, Mennella TA, Sawa C, Berthelet S, Krogan NJ, Wolek A, Podolny V, Carpenter LR, Greenblatt JF, Baetz K, Buratowski S.
 Genes Dev. 2006 Mar 15;20(6):660-5. [PMID: 16543219]
 [4] Chromosome-wide Rad51 spreading and SUMO-H2A.Z-dependent chromosome fixation in response to a persistent DNA double-strand break.
 Kalocsay M, Hiller NJ, Jentsch S.
 Mol Cell. 2009 Feb 13;33(3):335-43. [PMID: 19217407] 

Functional Description

 Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. This variant is enriched at promoters, it may keep them in a repressed state until the appropriate activation signal is received. Near telomeres, it may counteract gene silencing caused by the spread of heterochromatin proteins. Required for the RNA polymerase II and SPT15/TBP recruitment to the target genes. Involved in chromosome stability. 

Sequence Annotation

 REGION 98 108 Interaction with VPS72.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 4 4 N6-acetyllysine.
 MOD_RES 9 9 N6-acetyllysine.
 MOD_RES 11 11 N6-acetyllysine.
 MOD_RES 15 15 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Activator; Chromatin regulator; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Nucleosome core; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 134 AA 

Protein Sequence

Gene Ontology

 GO:0000790; C:nuclear chromatin; IDA:SGD.
 GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IGI:SGD.
 GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IMP:SGD.
 GO:0006334; P:nucleosome assembly; IEA:InterPro.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR002119; Histone_H2A. 

Pfam

 PF00125; Histone 

SMART

 SM00414; H2A 

PROSITE

 PS00046; HISTONE_H2A 

PRINTS

 PR00620; HISTONEH2A.